"VWR"
EDTA disodium salt dihydrate for biotechnology
Supplier: VWR
A reversible metalloprotease inhibitor useful for chelating metal ions.
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2-Mercaptoethanol ≥99%, Proteomics Grade for electrophoresis, for molecular biology
Supplier: VWR
2-Mercaptoethanol ≥99%, Proteomics Grade for electrophoresis, for molecular biology
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Bovine Rnase A (from Pancreas)
Supplier: VWR
Ribonuclease A is an endoribonuclease that efficiently hydrolyzes RNA contaminants in DNA preparations from tissue or bacterial cell cultures. RNase A is used for a variety of molecular biology applications, most commonly for the hydrolysis of RNA that contaminates DNA preparations. This enzyme is used during plasmid purification without nicking or degrading plasmid.
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Leupeptin
Supplier: VWR
A reversible inhibitor of cysteine proteases and serine proteases resembling trypsin
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L(+)-Cysteine hydrochloride monohydrate, high purity
Supplier: VWR
L(+)-Cysteine hydrochloride monohydrate, high purity
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Phenol ≥99% saturated solution in water, Biotechnology Grade, buffered
Supplier: VWR
Packaged at pH 6.6, this mixture is provided with a supplemental alkaline buffer that
allows the researcher to easily increase the pH to 7.9 for DNA purification. When mixed with Chloroform:Isoamyl Alcohol (24:1), it makes an optimized reagent for DNA purification. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.
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3,3',5,5'-Tetramethylbenzidine (TMB), liquid, Biotechnology Grade 1 component
Supplier: VWR
Ready-to-use sensitive substrate for the detection of horseradish peroxidase activity
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VWR® Protease Inhibitor Cocktails
Supplier: VWR
20X Protease Inhibitor Cocktail, Bacterial, VWR®
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Glutaraldehyde 50% in aqueous solution Reagent Grade, Reagent Grade
Supplier: VWR
Glutaraldehyde 50% in aqueous solution Reagent Grade, Reagent Grade
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Iodoacetamide ≥98%, Proteomics Grade
Supplier: VWR
Iodoacetamide ≥98%, Proteomics Grade
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Phenol ≥99.0% saturated with buffer pH 4.5
Supplier: VWR
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.
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n-Dodecyl-β-D-maltoside ≥99.0%, high purity
Supplier: VWR
Non-ionic - containing no charge (polyoxyethylenes or alkyl glucosides).
Non-ionic detergents are suited for solubilizing membrane proteins without altering biological activity.
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Polyethylene glycol 3,350, Biotechnology Grade
Supplier: VWR
Polyethylene glycol 3,350, Biotechnology Grade for biotechnology
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VWR® Loading Dye Base 50X
Supplier: VWR
Loading Dye Base, 50X is used for custom loading buffer preparation.
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