264 Results for: "Titration Solutions"

Supplier: MilliporeSigma

Supplier: MP Biomedicals

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to the appropriate pH.

Supplier: MP Biomedicals

Hydroxynaphthol blue, disodium salt is used for determining the endpoint in metal complexation titrations.

Supplier: Adipogen

TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (lambdamax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.

Supplier: Aqua Solutions

Supplier: MilliporeSigma

Supplier: MP Biomedicals

Calcein is used as an Indicator for the determination of calcium by titration with EDTA. Suitable for fluorometric determination of calcium.

Supplier: MP Biomedicals

Citric acid is a key metabolic intermediate. Citrate is the starting point of the tricarboxylic acid cycle. Its concentration also coordinates several other metabolic pathways. Citric acid can form complexes with various cations, particularly with iron and calcium. In animals, citric acid improves the utilization of nutritional calcium. Citric acid is produced commercially by fermentation of carbohydrates derived from corn starch and from beet molasses.
Citric Acid, Trisodium Salt, Dihydrate is used as a substrate for citrate lyase, a buffer component; an anticoagulant. For anticoagulation use it is typically used at a concentration of approximately 0.129 M (i.e. for 4.5 mL blood use 16.0 mg sodium citrate and 2.1 mg citric acid).
To make a sodium citrate buffer use equimolar concentrations (typically approximately 0.05 M concentration) of citric acid, free acid and sodium citrate. Add equal volumes of each solution and titrate to the desired pH.
Room Temperature

Supplier: MP Biomedicals

Calcein is an indicator for the determination of calcium by titration with EDTA. The excitation wavelength and emission wavelength are 495/515 nm.

Supplier: Adipogen

Water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range and frequently used in cell biology laboratories. Widely used as cell tissue culture pH marker. A small amount of phenol red added to the growth medium will have a pink-red color under normal conditions. Typically, 15 mg/l are used for cell culture. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. This usually results in an acidification of the medium, turning it yellow. The waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. This color change is an indication that even in the absence of contamination, the medium needs to be replaced. Shown to be a weak estrogen mimic (possible through inpurities), which can enhance the growth of cells that express the estrogen receptor in cell cultures. Might be useful as a differentiation factor.

Supplier: Adipogen

A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.

Supplier: MP Biomedicals

2-Hydroxy-1-(2-hydroxy-4-sulfo-1-naphthylazo)-3-naphthoic acid is a useful indicator for calcium titrations with EDTA in the presence of magnesium.

Supplier: Aqua Solutions

Bromine number titration solvent ASTM 1159 & ASTM D 2710

Supplier: Ricca Chemical

Eosin Y (yellowish) 0.5% (w/v) in aqueous solution USP Test Solution (TS) adsorption indicator for argentometric titrations

Supplier: MP Biomedicals

Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.
Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.

Supplier: MilliporeSigma

Supplier: MilliporeSigma

Supplier: Aqua Solutions

TITRATION SOLUTION 4L

Supplier: Aqua Solutions

SOLVENT TITRATION 4 L FOR CHLORIDES

Supplier: Aqua Solutions

TITRATION SOLVENT FOR CHLORIDES 4L

Supplier: Aqua Solutions

TITRATION SOLVENT 2:1 PHCL ACETIC 4L

Supplier: Aqua Solutions

ACIDIC TITRATION SOLVENT CLEAR 20L DISTL

Supplier: Aqua Solutions

ALKALINE TITRATION SOLVENT 4L GAS/NAPTHA

Supplier: Photovolt Instruments

Titrator

Supplier: Hach

For determination of alkalinity by Digital Titrator titration.

Supplier: MilliporeSigma

Supplier: MilliporeSigma

Supplier: Honeywell Research Chemicals

Supplier: Ricca Chemical

pH Indicator solution universal pH 2-10

Supplier: MilliporeSigma