"Test Lead"

Supplier: Promega Corporation

The cAMP-Glo Assay is a homogeneous, bioluminescent and high-throughput assay for measuring cAMP levels in cells

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Rockland Immunochemical

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKa, IKKb and IKKe kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.

Supplier: Prosci

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001).

Supplier: Ansell Healthcare

Sleeve protectors are manufactured from breathable, microporous film laminate, which is an excellent particle barrier (100% dry particle holdout between 0.3–0.5µm)

Supplier: Rockland Immunochemical

The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.

Supplier: Eco Lab

The Bioquell Qube is a configurable isolator integrated with Bioquell’s Hydrogen Peroxide Vapor technology. From its unique design to customizable features, the Bioquell Qube helps ensure your aseptic workspace needs are met for a productive working environment.

Supplier: Rockland Immunochemical

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.

Supplier: Rockland Immunochemical

X-linked ectodysplasin-A2 receptor (XEDAR) is a recently isolated member of the tumor necrosis factor receptor family that is highly expressed during embryonic development and binds to ectodysplatin-A2 (EDA-A2). Two predominantly expressed isoforms, XEDAR-s and XEDAR-L, differ by only a 21-amino region at the juxtamembrane region of the cytoplasmic domain. Neither isoform possesses a death domain and both have been shown to act mainly through TRAF3 and TRAF6 to activate the NF-kB and JNK pathways. Cells transfected with XEDAR and treated with EDA-A2 cause the assembly of a secondary complex containing FADD, caspase-8 and caspase-10, leading to the activation caspase-8 and caspase-3, and finally apoptosis. The EDA-A2-induced apoptosis is dependent on caspase-9 activation, as various pharmacological and genetic inhibitors of caspase-8 blocked apoptosis following EDA-A2 treatment.

Supplier: Desco

Statshield® Dissipative 6.5 mil Moisture Barrier Bags are designed for ESD/EMI/RFI protection.

Supplier: Rockland Immunochemical

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism (i.e., the metabolism of environmental mutagens and carcinogens) and may play a role in susceptibility to cancer. More recent experiments have suggested that differential expression of GSTP1 also contributes to the sensitivity of xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders such as Parkinson's disease. CpG island hypermethylation of the GSTP1 promoter leading to the silencing of the GSTP1 gene has also been linked to cancer.

Supplier: Prosci

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).

Supplier: Rockland Immunochemical

The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Supplier: MilliporeSigma

Discovery® BIO Wide Pore reversed-phase columns satisfy the need for efficiency, selectivity, scalability, and reproducibility for RP HPLC analyses of proteins, peptides, and small biomolecules.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.

Supplier: Rockland Immunochemical

The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B. NK3R antibody does not recognize NK1R or NK2R.