4629 Results for: "Tantalum(V)+ethanolate"
Sodium chloride ≥99.5%, white crystalline powder for molecular biology
Supplier: MP Biomedicals
Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water 10), density gradients 3,6, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. A salt-ice mixture in the ratio of approximately 33 g sodium chloride to 100 g ice (at -1°C) will drop in temperature to as low as -21°C, depending on the rate of stirring and the size of the ice chunks.
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Phorbol-12-myristate-13-acetate ≥99%, colorless material
Supplier: MP Biomedicals
PMA (Phorbol-12-Myristate-13-Acetate) is a diester of phorbol and is a tumor promoting compound extracted from croton oil. It is a reversible, highly potent protein kinase C (PKC) activator in vitro and in vivo at nM concentrations. PMA’s effects on PKC are attributed to its similarity to diacylgylerol, a natural activator of PKC.
PMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.
Potent tumor promoter; activates protein kinase C in vivo and in vitro.
Store at -20 °C. Protect from light.
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EconoTaq® PLUS 2X PCR Master Mix, Biosearch Technologies
Supplier: Lucigen
EconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer superior performance, convenience, reliability, and value for routine PCR
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Sodium cholate ≥98.5%, white powder
Supplier: MP Biomedicals
Cholic acid is a biochemical solubilizing agent (a non-ionic, non-denaturing detergent). Occurs inconjugation with glycine or taurine in bile of most vertebrates.
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rProtein A Sepharose™ Fast Flow Affinity Purification Media, Cytiva
Supplier: Cytiva
rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.
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Dithiothreitol (DTT, Cleland's reagent) ≥99.5%, white powder for electrophoresis
Supplier: MP Biomedicals
DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.
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NanoPhotometer® N50-TOUCH UV/Visible Spectrophotometer for NanoVolume Applications, Implen
Supplier: IMPLEN U.S.A. INC
Implen has become the leading expert for innovative, high-quality spectroscopy instruments and the NanoPhotometer® is trusted by thousands of researchers worldwide.
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Glycine ≥99% for molecular biology
Supplier: MP Biomedicals
Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.
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Brefeldin A ≥98%, white powder for molecular biology
Supplier: MP Biomedicals
Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells.
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.
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GelRed® Prestain Plus 6X DNA Loading Dye, Biotium
Supplier: Biotium
6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.
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Total RNA Plant Kits, IBI Scientific
Supplier: IBI Scientific
The Total RNA Mini and Maxi Kits for Plants provide a simple and fast method to isolate total RNA from plant tissue and cells
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Sera-Xtracta™ HMW DNA Kit
Supplier: Cytiva
Sera-Xtracta™ HMW DNA Kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood.
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96-Well Total RNA Kits (Plant Tissue and Cells), IBI Scientific
Supplier: IBI Scientific
The 96-Well Total RNA Kit (Plant) was specially designed for high-throughput purification of total RNA from plant tissue and cells
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Hi-Speed Mini Plasmid Kits, IBI Scientific
Supplier: IBI Scientific
IBI's Hi-Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from bacterial cultures
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Genomic DNA Tissue Cell Kits, IBI Scientific
Supplier: IBI Scientific
Kits are designed specifically for purifying total DNA (including genomic, mitochondrial, and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab, and amniotic fluid
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Gel/PCR DNA Fragment Extraction Kits (Large and Small Fragment), IBI Scientific
Supplier: IBI Scientific
The Large DNA Fragment Extraction Kits are designed to recover large DNA fragments (>8Kb) from an agarose gel
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E.Z.N.A.® Blood DNA Kit, Omega Bio-tek
Supplier: Omega Bio-Tek
The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1–250 µL of fresh and frozen anticoagulated whole blood
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Trypan blue 0.4% in PBS, dark blue solution
Supplier: MP Biomedicals
Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxicity and proliferation assays.
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
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miRNA Isolation Kit, IBI Scientific
Supplier: IBI Scientific
The miRNA Isolation kits are designed for the purification of microRNA (miRNA) and other small cellular RNAs from tissue samples and cultured cells
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SV Total RNA Isolation Start-Up Kit, 110 V Electrical (250 Preparations, Manifold and Free Vacuum Pump), 1 Each, Promega
Supplier: Promega Corporation
A fast, simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.
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Viral Nucleic Acid Extraction Kits, IBI Scientific
Supplier: IBI Scientific
IBI's Viral Nucleic Acid Extraction Kit was designed specifically for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids, and the supernatant of viral infected cell cultures
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RNA Clean & Concentrator™ Kits, Zymo Research
Supplier: Zymo Research
The RNA Clean & Concentrator™ (RCC™) kits facilitate the efficient removal of RNA polymerases, ligases, and RNA modifying enzymes.
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RNAstorm™ RNA Isolation Kit, Biotium
Supplier: Biotium
The RNAstorm™ kit provides a convenient and efficient method of isolating RNA from fresh or frozen cells or tissue samples.
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ReliaPrep FFPE gDNA Miniprep System, Promega
Supplier: Promega Corporation
The ReliaPrep FFPE gDNA Miniprep System provides a complete, all-inclusive method for purifying quality genomic DNA from formalin-fixed paraffin-embedded tissue without using hazardous solvents or overnight digestion.
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ReliaPrep RNA Tissue Miniprep System, Promega
Supplier: Promega Corporation
The ReliaPrep RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes from very small amounts of input material.
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96-Well PCR Clean-Up Kits, IBI Scientific
Supplier: IBI Scientific
The IBI high throughput 96-Well PCR Clean-Up Kit is designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions using an efficient 96-well binding plate system.
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Mini Genomic DNA Kit (Plant/Fungi), IBI Scientific
Supplier: IBI Scientific
Kits provide a fast and economical method for the purification of total DNA (including genomic, mitochondrial, and chloroplast DNA) from plant tissue and cells
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gMAX Genomic DNA Kit, IBI Scientific
Supplier: IBI Scientific
IBI's gMax MINI Genomic DNA Extraction/Purification Kit provides a fast and efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood (fresh/frozen), tissue, buccal swab, and amniotic fluid
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ReliaPrep Blood gDNA Miniprep System, Promega®
Supplier: Promega Corporation
The ReliaPrep Blood gDNA Miniprep System provides a complete, ready-to-use method for purification of gDNA from up to 200ul of blood or body fluid, consistently isolating pure, intact gDNA without the use of alcohol washes or precipitations.