Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched non-human primate CD4+ T cells

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human T cells.

Supplier: Stemcell Technologies

Immunomagnetic negative selection kit

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched rat CD4+ T cells

Supplier: Stemcell Technologies

Cell dissociation reagent.

Supplier: Stemcell Technologies

The Hypoxia Incubator Chamber is for generation of a hypoxic environment for tissue culture. This is a self-contained and sealed chamber that fits inside existing laboratory incubators.

Supplier: Stemcell Technologies

A non-integrating, self-replicating RNA reprogramming vector for generating iPS cells.

Supplier: Stemcell Technologies

Kit including BrainPhys™ neuronal medium, SM1 meuronal supplement and NeuroCult™ neuronal plating medium for culture of primary-derived neurons.

Supplier: Stemcell Technologies

Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells.

Supplier: Stemcell Technologies

Immunomagnetic positive selection of mouse cells labeled with APC-conjugated antibodies.

Supplier: Stemcell Technologies

Immunomagnetic negative selection of human pan-B cells

Supplier: Stemcell Technologies

15 ml centrifuge tube with an insert for density gradient centrifugation

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched mouse naive CD4+ T cells

Supplier: Stemcell Technologies

Medium for detection of CFU-F and expansion of human mesenchymal stem cells.

Supplier: Stemcell Technologies

Animal component-free medium and attachment substrate for human mesenchymal stem cells.

Supplier: Stemcell Technologies

Medium for dissociation of mouse spleen.

Supplier: Stemcell Technologies

Immunomagnetic positive selection of CD138+ cells (e.g. plasma cells) from mouse splenocytes or other tissues.

Supplier: Stemcell Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).

Supplier: Stemcell Technologies

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux and Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of the placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam and Ghosh). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam and Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).

Supplier: Stemcell Technologies

Immunomagnetic positive selection of particle-free mouse cells labeled with biotinylated antibodies.

Supplier: Stemcell Technologies

For preparation of collagen gels and for coating cell culture surfaces - 35 ml.

Supplier: Stemcell Technologies

For differentiation of human PSCs into the hematopoietic lineage.

Supplier: Stemcell Technologies

For dissociation of hPSC-derived cardiomyocytes.

Supplier: Stemcell Technologies

For culture, expansion, and drug screening of chronic and acute myeloid leukemia cells.

Supplier: Stemcell Technologies

Culture medium for plating dissociated primary tissue-derived neurons for improved survival.

Supplier: Stemcell Technologies

Defined, serum-free medium for generation and culture of embryoid bodies using AggreWell™ plates.

Supplier: Stemcell Technologies

Interleukin 6 (IL-6) is a pleiotropic growth factor with the wide range of biological activities in immune regulation, hematopoiesis, and oncogenesis. IL-6 is produced by a variety of cell types including T cells, B cells, monocytes and macrophages, fibroblasts, hepatocytes, vascular endothelial cells, and various tumor cell lines. On its own or in combination with other factors such as IL-2 and interferon-γ, IL-6 stimulates the proliferation of B cells, T cells, and hybridoma cells (Hirano et al.; Mihara et al.; Tanaka et al). In combination with cytokines such as IL-3, GM-CSF and SCF, IL-6 has been shown to promote hematopoietic progenitor cell proliferation and differentiation in vitro. IL-6 signals through a cell surface type I cytokine receptor complex consisting of the ligand-binding IL-6α (CD126) and the signal-transducing gp130 subunits. The binding of IL-6 to its receptor system includes activation of JAK/STAT signaling pathway (Mihara et al.; Peters et al.; Tanaka et al.).

Supplier: Stemcell Technologies

Column-free immunomagnetic positive selection of human B cells for HLA analysis.

Supplier: Stemcell Technologies

cGMP, stabilised feeder-free maintenance medium for human ES and iPS cells.

Supplier: Stemcell Technologies

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). It has been suggested that PDGF-AA is an important autocrine regulator of vascular endothelial growth factor (VEGF) expression in non-small cell lung carcinomas (Shikada et al.). PDGF-AA also mediates proliferation of oligodendrocyte progenitor cells and oligodendrocyte lineage differentiation through the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) (Hu et al.). PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells (Piao et al.).