"Stemcell Technologies"

Supplier: Stemcell Technologies

Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of megakaryocytes and has an important role in regulating the level of circulating platelets in vivo. TPO also promotes the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitor cells. It is commonly used with other cytokines such as stem cell factor (SCF) and Flt3/Flk-2 Ligand to promote expansion of primitive hematopoietic cells in culture (Hitchcock andamp; Kaushansky). The TPO receptor, c-Mpl, is expressed at all stages of megakaryopoiesis, from hematopoietic stem and progenitor cells to mature platelets.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human CD56 (NCAM), APC­conjugated.

Supplier: Stemcell Technologies

Animal component-free non-specific protease.

Supplier: Stemcell Technologies

Kit including BrainPhys™ Neuronal Medium, SM1 Neuronal Supplement, and N2 Supplement-A for culture of ES/iPS cell-derived neurons.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched mouse neutrophils

Supplier: Stemcell Technologies

Coating for cultureware.

Supplier: Stemcell Technologies

Membrane-permeable fluorescent probe for the detection of live neurons - 0.1 ml.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human CD56 (NCAM), Alexa Fluor® 488-conjugated.

Supplier: Stemcell Technologies

Immunomagnetic positive selection of human CD3+ cells (e.g. T cells).

Supplier: Stemcell Technologies

Interleukin 6 (IL-6) is a pleiotropic growth factor with a wide range of biological activities in immune regulation, hematopoiesis, and oncogenesis. IL-6 is produced by a variety of cell types including T cells, B cells, monocytes and macrophages, fibroblasts, hepatocytes, vascular endothelial cells, and various tumor cell lines. On its own or in combination with other factors such as IL-2 and interferon-γ, IL-6 stimulates the proliferation of B cells, T cells, and hybridoma cells (Nordan et al.; Van Snick et al.; Gauldie et al.; Mihara et al.; Tanaka et al). In combination with cytokines such as IL-3, GM-CSF, and SCF, IL-6 has been shown to promote hematopoietic progenitor cell proliferation and differentiation in vitro. IL-6 signals through a cell surface type I cytokine receptor complex consisting of the ligand-binding IL-6α (CD126) and the signal-transducing gp130 subunits. The binding of IL-6 to its receptor system includes activation of the JAK/STAT signaling pathway (Mihara et al.; Peters et al; Tanaka et al.).

Supplier: Stemcell Technologies

Immunomagnetic isolation of human granulocytes directly from whole blood

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human leukocytes from whole blood.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human eosinophils.

Supplier: Stemcell Technologies

Immunomagnetic isolation of naïve B cells directly from whole blood

Supplier: Stemcell Technologies

Interleukin 1 beta (IL-1β) is synthesized as an inactive precursor protein or pro-IL-1β. This precursor is cleaved intracellularly by caspase 1 (IL-1β convertase) to form the active form of the protein that is later secreted (Allan et al.). IL-1β binds to IL-1 receptor and activates intracellular signaling via the MAPK or NF-κB pathway. IL-1β is released by monocytes, tissue macrophages, and dendritic cells in response to infection or injury and induces expression of acute-phase proteins. It also promotes the infiltration of inflammatory and immunocompetent cells from the circulation into the extravascular space and affected tissues, by stimulating the expression of adhesion molecules on endothelial cells. IL-1β also affects other immune cells; for example, it co-stimulates T cell functions together with an antigen or mitogen. It also stimulates Th17 differentiation and B cell proliferation in an IL-6-dependent manner.

Supplier: Stemcell Technologies

Defined medium kit for transgene-free induction of reset naïve human pluripotent stem cells - 1 Kit