671 Results for: "Stemcell Technologies"

Supplier: STEMCELL Technologies

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).

Supplier: STEMCELL Technologies

Support cell attachment, growth, and proliferation with CellAdhere™ Type I Collagen, Human, Lyophilized. The most abundant type of collagen in the human body, type I collagen is a natural extracellular matrix protein that provides structural support and mimics physiological conditions, making it an ideal cultureware coating surface to support cell attachment in 2D applications.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of particle-free human CD4+ cells.

Supplier: STEMCELL Technologies

For digestion of DNA.

Supplier: STEMCELL Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

Supplier: STEMCELL Technologies

Animal component-free collagenase for the digestion of native collagen fibrils.

Supplier: STEMCELL Technologies

Coating material for the differentiation of lymphoid cells.

Supplier: STEMCELL Technologies

A specialized media kit for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes.

Supplier: Stemcell Technologies

Serum-free methylcellulose-based medium with recombinant cytokines (including EPO) for mouse erythroid progenitor cells.

Supplier: STEMCELL Technologies

Interferon-alpha (IFN-α) is a type I interferon, produced by virus-infected cells, and is released as a soluble factor to initiate antiviral responses (Isaacs and Lindenmann). IFN-α2 is the most potent IFN-α used in fundamental research and in most clinical applications. The best-known IFN-α2 subvariants, 2A and 2B, differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (von Gabain et al.). Type I IFNs exert potent antitumor activity by increasing the cytotoxic activity of NK and T cells, as well as by inhibiting the proliferation of cancer cells (Paul et al.). Additionally, it has been shown that proinflammatory IFN-α modulates the function of B cells in patients with systemic lupus erythematosus (Chang et al.), and pegylated forms of IFN-alpha 2A and 2B have implications in the treatment of hepatitis C (Foster et al.).

Supplier: STEMCELL Technologies

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic growth factor that promotes proliferation, motility, survival, and differentiation. HGF binds and promotes the dimerization and activation of the receptor tyrosine kinase c-MET, and stimulates PI3K/AKT, FAK, JNK, and ERK1/2 signaling (Organ and Tsao). HGF stimulates migration of cells during embryogenesis, induces cell motility and scattering of epithelial cells, and regulates epithelial-mesenchymal transition. It is a chemoattractant for motor neurons, and regulates the development of sensory, sympathetic, parasympathetic, and cortical neurons as well as the proliferation of oligodendrocytes and glial development. Additionally, HGF is cytoprotective and regulates liver growth and regeneration (Nakamura et al.).

Supplier: STEMCELL Technologies

Serum-free culture kit for expansion of human B cells.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched mouse NK cells

Supplier: STEMCELL Technologies

Serum- and xeno-free kit for differentiation and expansion of human pluripotent stem cell-derived endothelial cells.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of human CD34+ cells (e.g. hematopoietic, stem/progenitor cells)

Supplier: STEMCELL Technologies

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knots (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4); GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen and Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increases their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor; MSPP-78005), BrainPhys™ Neuronal Medium (MSPP-05790), and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched rat B cells

Supplier: STEMCELL Technologies

Dilution buffer for matrix proteins.

Supplier: STEMCELL Technologies

Immunodensity isolation of untouched CD8+ T cells.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human B cells.

Supplier: STEMCELL Technologies

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells.

Supplier: STEMCELL Technologies

Serum-free medium for differentiation of human monocytes to macrophages.

Supplier: STEMCELL Technologies

Single flow meter is specially designed to control the gas flow in hypoxia incubator chamber to generate a hypoxic environment for tissue culture.

Supplier: STEMCELL Technologies

Enzyme-free reagent for the selective detachment of neural rosettes.

Supplier: STEMCELL Technologies

Serum-free and xeno-free medium for the expansion of human T cells.

Supplier: STEMCELL Technologies

Complete medium for differentiating mouse mesenchymal stem cells and embryonic fibroblasts into osteoblasts.

Supplier: STEMCELL Technologies

Serum-free medium for culture and expansion of hematopoietic cells.

Supplier: STEMCELL Technologies

Serum-free medium for culture and expansion of hematopoietic cells.

Supplier: STEMCELL Technologies

Methylcellulose-based medium with recombinant cytokines for human cells. 1×100 ml bottle.

Supplier: STEMCELL Technologies

Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface.