671 Results for: "Stemcell Technologies"

Supplier: STEMCELL Technologies

Kit including BrainPhys™ neuronal medium, SM1 meuronal supplement and NeuroCult™ neuronal plating medium for culture of primary-derived neurons.

Supplier: STEMCELL Technologies

Deoxyribonuclease (DNase) I solution is a cell dissociation reagent useful to reduce or prevent the clumping of concentrated or cryopreserved cell suspensions following thawing.

Supplier: STEMCELL Technologies

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils, and basophils) and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and used to treat chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).

Supplier: STEMCELL Technologies

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions.

Supplier: STEMCELL Technologies

Maturation kit for the generation of astrocytes from human ES and iPS cell-derived astrocyte precursors.

Supplier: STEMCELL Technologies

Serum-free culture supplement for expansion and differentiation of human granulocytes.

Supplier: STEMCELL Technologies

Defined supplement for single-cell cloning of human ES and iPS cells.

Supplier: STEMCELL Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched mouse naive CD4+ T cells

Supplier: STEMCELL Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.). This product is animal component-free.

Supplier: STEMCELL Technologies

Defined, feeder-free maintenance medium for human ES and iPS cells.

Supplier: STEMCELL Technologies

Animal component-free medium and attachment substrate for human mesenchymal stem cells.

Supplier: STEMCELL Technologies

Medium for dissociation of mouse spleen.

Supplier: STEMCELL Technologies

Insulin-like growth factor 1 (IGF-I) is a polypeptide which belongs to the family of insulin-like growth factors that are similar in molecular structure to proinsulin. IGF-I binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway; it also activates ERK1/2 signaling. IGF-I is required for embryonic development, and it is produced mainly in liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells (Wang et al.). Together with interleukin 3 (IL-3), IGF-I stimulates differentiation and proliferation of myeloid cells, and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched rat CD4+ T cells

Supplier: STEMCELL Technologies

15 ml centrifuge tube with an insert for density gradient centrifugation

Supplier: STEMCELL Technologies

Medium for detection of CFU-F and expansion of human mesenchymal stem cells.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of CD138+ cells (e.g. plasma cells) from mouse splenocytes or other tissues.

Supplier: STEMCELL Technologies

Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells.

Supplier: STEMCELL Technologies

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux and Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of the placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam and Ghosh). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam and Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).

Supplier: STEMCELL Technologies

Myeloma and hybridoma culture medium (serum-containing).

Supplier: STEMCELL Technologies

For preparation of collagen gels and for coating cell culture surfaces - 35 ml.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of mouse cells labeled with APC-conjugated antibodies.

Supplier: STEMCELL Technologies

Defined cell culture medium for expansion of reset naïve human embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Supplier: STEMCELL Technologies

Serum- and BPE-free medium for expansion of primary human airway epithelial cells.

Supplier: STEMCELL Technologies

For culture, expansion, and drug screening of chronic and acute myeloid leukemia cells.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human pan-granulocytes

Supplier: STEMCELL Technologies

For dissociation of hPSC-derived cardiomyocytes.

Supplier: STEMCELL Technologies

Culture medium for plating dissociated primary tissue-derived neurons for improved survival.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched non-human primate CD4+ T cells