671 Results for: "Stemcell Technologies"

Supplier: STEMCELL Technologies

Immunomagnetic isolation of eosinophils directly from whole blood

Supplier: STEMCELL Technologies

Basic fibroblast growth factor (bFGF) is a prototypic member of the fibroblast growth factor family. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman and Klagsbrun; Kimelman and Kirschner) and are involved in a variety of biological processes including cell proliferation, differentiation, survival, and apoptosis (Folkman and Klagsbrun; Klagsbrun; Rifkin and Moscatelli). bFGF has the β-trefoil structure (Ponting and Russell), binds to the four FGF receptor (FGFR) family members, and activates JAK/STAT, PI3K, ERK1/2, and other receptor tyrosine kinase (RTK) signaling pathways. It supports the maintenance of undifferentiated human pluripotent stem cells (Xu et al.; Kang et al.), stimulates human pluripotent stem cells to form neural rosettes (Zhang et al.), and improves proliferation of human mesenchymal stem cells and enhances chondrogenic differentiation (Solchaga et al.). This version of bFGF is the full-length bFGF protein encoded by the human FGF2 gene consisting of 154 amino acid residues. This product is animal component-free.

Supplier: STEMCELL Technologies

cGMP, enzyme-free cell dissociation reagent.

Supplier: STEMCELL Technologies

Reliably isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™, a cost-effective alternative to Ficoll-Paque™.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human CD8+ T cells.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human peripheral blood mononuclear cells from whole blood

Supplier: STEMCELL Technologies

cGMP, feeder-free maintenance medium for human ES and iPS cells.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of particle-free mouse cells labeled with PE-conjugated antibodies.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of human EpCAM+ cells (e.g. mammary epithelial cells).

Supplier: STEMCELL Technologies

Pre-aliquoted methylcellulose-based medium with recombinant cytokines for human cells. 24x3ml vials.

Supplier: STEMCELL Technologies

Immunodensity isolation of untouched NK cells.

Supplier: STEMCELL Technologies

Serum-free culture supplement for expansion of human hematopoietic cells.

Supplier: STEMCELL Technologies

Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids.

Supplier: STEMCELL Technologies

Kit for generating iPS cells using ReproRNA™-OKSGM, a non-integrating, self-replicating RNA reprogramming vector.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of NK cells directly from whole blood

Supplier: STEMCELL Technologies

Immunomagnetic isolation of mouse CD4+CD25+ regulatory T cells.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched mouse plasmacytoid dendritic cells (pDC)

Supplier: STEMCELL Technologies

Immunomagnetic negative selection kit.

Supplier: STEMCELL Technologies

Bone morphogenetic protein 4 (BMP-4) is a member of the highly conserved transforming growth factor β (TGF-β) superfamily. Mature BMP-4 is a disulfide-linked homodimeric protein consisting of two 116-amino-acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides (Xiao et al.). BMP-4 binds to type I and type II receptors on cells. This binding results in the phosphorylation of receptor 1, which in turn results in the phosphorylation of Smad proteins, which then go on to act as transcription factors (Zhang et al.). BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells (Chadwick et al.; Graham et al.; Jones et al.; Lengerke et al.; Zhang et al.).

Supplier: STEMCELL Technologies

For depletion of red blood cells from fresh blood samples and isolation of nucleated cells.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of CD90.2+ cells from mouse splenocytes or other tissues.

Supplier: STEMCELL Technologies

Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor (Gaffen and Liu). It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival (Ma et al.). The majority of IL-2 is secreted by activated CD4+ and CD8+ T cells, although B cells and dendritic cells were found to produce IL-2 in small amounts. IL-2 downregulates immune responses to prevent autoimmunity during thymic development, influences the development of CD4+CD25+ regulatory T cells, and affects development of follicular helper T cells. IL-2 also controls inflammation by inhibiting Th17 differentiation (Banchereau et al.). High IL-2 levels in serum are associated with progression of scleroderma, rheumatoid arthritis, and gastric and non-small cell lung cancer, though no known disease can be directly attributed to the lack or excess of IL-2 (Gaffen and Liu).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched mouse pan-dendritic cells (DCs)

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human CD4+ T cells.

Supplier: STEMCELL Technologies

The EasySep™ 'The Big Easy' magnet is designed for cell separation procedures using EasySep™ reagents when processing a large number of cells, or when using RoboSep™.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of mouse naïve CD4+ T cells.

Supplier: STEMCELL Technologies

The EasySep™ Easy 50 magnet is designed for cell separation procedures using selected EasySep™ reagents when processing a large number of cells (up to 4×10⁹ cells).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human plasmacytoid dendritic cells (pDC)

Supplier: STEMCELL Technologies

Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin supports maintenance of undifferentiated human embryonic stem cells in vitro, and can be used to prevent spontaneous differentiation in the short term (Chaturvedi et al.). Noggin is essential for development of structures derived from ectoderm embryonic somite, skeletal patterning, and neurogenesis in vivo. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al.), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al.).

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of human CD14+ cells (e.g. monocytes).