"Stemcell Technologies"

Supplier: Stemcell Technologies

Brain-derived neurotrophic factor (BDNF), like nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4), is a member of the NGF family of neurotrophins, which are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems (Minichiello and Klein; Minichiello et al.). BDNF binds with high affinity to the tropomyosin receptor kinase B (TrkB), and activates AKT and ERK pathways (Mattson et al.). It is expressed in the hippocampus, cortex, and synapses of the basal forebrain. BDNF acts as a survival factor for human embryonic stem cells when plated on either feeder cells or Corning® Matrigel® (Pyle et al.). BDNF regulates synaptic transmission and plasticity at adult synapses in the central nervous system, and contributes to adaptive neuronal responses including long-term potentiation, long-term depression, certain forms of short-term synaptic plasticity, and homeostatic regulation of neuronal excitability (Reichardt). It also has a role in neurogenesis by promoting survival and growth of dorsal root ganglion cells, and hippocampal and cortical neurons (Binder and Scharfman). BDNF, together with glial cell line-derived neurotrophic factor (GDNF) and other supplements, is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Brafman).

Supplier: Stemcell Technologies

Mouse monoclonal IgG2b, kappa isotype control antibody, Alexa Fluor® 488-conjugated.

Supplier: Stemcell Technologies

Serum-free culture supplement for expansion and differentiation of human monocytes.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human myeloid dendritic cells (mDCs).

Supplier: Stemcell Technologies

Isolate untouched and highly purified CD8+ T cells from mouse splenocytes by immunomagnetic negative selection. When using single-cell suspensions from other tissue types, this kit may require optimization.

Supplier: Stemcell Technologies

Serum-free methylcellulose-based medium with recombinant cytokines for human ES and iPS cell-derived cells.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched mouse T cells

Supplier: Stemcell Technologies

cGMP, serum-free, and xeno-free medium for the expansion of human T cells.

Supplier: Stemcell Technologies

Defined, animal component-free medium for differentiation of human ES and iPS cells to multiple lineages.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human naïve B cells

Supplier: Stemcell Technologies

Immunomagnetic positive selection of CD8a+ cells from mouse splenocytes or other tissues.

Supplier: Stemcell Technologies

Immunomagnetic isolation of CD8+ T cells directly from whole blood

Supplier: Stemcell Technologies

Immunodensity isolation of untouched myeloid cells for lineage-specific chimerism analysis.

Supplier: Stemcell Technologies

Base methylcellulose medium for mouse cells.

Supplier: Stemcell Technologies

Serum-free media for generation and maturation of branching lung organoids from human ES and iPS cells.

Supplier: Stemcell Technologies

Serum-free and animal component-free culture supplement for expansion of human B cells.