3025 Results for: "Southern Biotechnology"

Supplier: Southern Biotechnology

Chicken IgY-HRP for use as a control in immunocytochemistry assays.

Supplier: Southern Biotechnology

Low Endotoxin/Azide Free Anti-Mouse IL-6 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, ELISA, ELISpot, multiplex, and neutralization assays.

Supplier: Southern Biotechnology

CD49f is the 125 kDa integrin α6 chain which associates noncovalently with the integrin β1 subunit (CD29) and with the integrin β4 (CD104) chain to form, respectively, the very late antigen-6 (VLA-6) and α6β4 integrin heterodimers. VLA-6 is expressed on thymocytes, T lymphocytes, monocytes, and platelets. CD49f/CD29 expression is upregulated on activated T lymphocytes and memory T cells. VLA-6 serves as the laminin receptor on platelets, monocytes, and T cells. VLA-6-mediated binding to laminin provides a costimulatory signal to T cells for activation and proliferation.

Supplier: Southern Biotechnology

The chicken Ia antigen is expressed on B cells, a subpopulation of the monocyte/macrophage lineage of cells, and mitogen-activated T cells. The molecule recognized by this antibody is similar in structure to the murine Ia antigens and human DR antigens.

Supplier: Southern Biotechnology

CD15 is a carbohydrate antigen found on the cell surface of neutrophils and monocytes, but not on basophils, platelets, lymphocytes, or erythrocytes. CD15 may function in adhesion as its ligand is L-selectin (CD62L).

Supplier: Southern Biotechnology

Biotin Anti-Chicken IgM antibody for use in flow cytometry and immunoprecipitation assays.

Supplier: Southern Biotechnology

Purified Anti-Human IgG2 Fc antibody for use in ELISA, flow cytometry, immunocytochemistry, western blotting, ELISpot, and multiplex assays.

Supplier: Southern Biotechnology

Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II transmembrane glycoprotein that is expressed as a heterodimer on the suppressor/cytotoxic subpopulation of peripheral T lymphocytes. It is present on approximately 76% of thymocytes, 14% of splenocytes, 1% of bone marrow cells, and 15% of peripheral blood lymphocytes. CD8 functions as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition.

Supplier: Southern Biotechnology

Swine Anti-Goat IgG(H+L)-Biotin antibody with minimal reactivity to human, mouse, and rat serum proteins for use in ELISA, flow cytometry, immunohistochemistry, and western blotting assays.

Supplier: Southern Biotechnology

Purified Goat Anti-Rat IgM antibody for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Supplier: Southern Biotechnology

Rabbit F(ab')2 Anti-Goat IgG(H+L)-HRP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.

Supplier: Southern Biotechnology

HRP Anti-Rat IgG1 antibody for use in ELISA, immunohistochemistry, and ELISpot assays.

Supplier: Southern Biotechnology

Porcine CD1 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It has a domain organization similar to that of MHC class I molecules and is expressed in association with β2-microglobulin. CD1 is found on B cells, macrophages, and immature thymocytes. There is evidence for a role of CD1 in presentation of lipids and peptides to T cells.

Supplier: Southern Biotechnology

Goat Anti-Rat IgG-PE antibody for use in flow cytometry assays.

Supplier: Southern Biotechnology

The monoclonal antibody BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues - 11-15% in peripheral blood, 22-23% in mesenteric lymph nodes, 79-87% in ileal Peyer’s patches (ILPP), 6-17 in spleen, and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by BB6-10A10 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.

Supplier: Southern Biotechnology

Human IgG-AP for use as a control in ELISA assays.

Supplier: Southern Biotechnology

FITC Anti-Mouse Kappa antibody for use in flow cytometry, immunohistochemistry, and western blotting assays.

Supplier: Southern Biotechnology

ApoScreen® Annexin V (SB Cat. No. 10040) is a recombinant protein that is expressed in E. coli. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.

Supplier: Southern Biotechnology

CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Supplier: Southern Biotechnology

CD3ε, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3ε is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex of mouse strains tested.

Supplier: Southern Biotechnology

CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.

Supplier: Southern Biotechnology

Mouse IgG2b-APC for use as a control in flow cytometry assays.

Supplier: Southern Biotechnology

Rat IgG2a-CY5 for use as a control in flow cytometry and immunohistochemistry assays.

Supplier: Southern Biotechnology

The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.

Supplier: Southern Biotechnology

CD24, also known as heat stable antigen, is a phosphatidylinositol-linked cell surface glycoprotein that is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+CD8+CD24+ thymocytes to either CD4+CD8-CD24- or CD4-CD8+CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P-selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes.

Supplier: Southern Biotechnology

CD79 is a disulfide-linked heterodimer composed of an α chain (CD79a) and a β chain (CD79b) that associates non-covalently with membrane immunoglobulin to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes first appearing on the surface at the pro-B cell stage and remaining through all stages of B cell differentiation prior to plasma cells. Crosslinking of the BCR leads to B cell activation. The monoclonal antibody CB3-1 reacts with the β chain of CD79.

Supplier: Southern Biotechnology

Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa

Supplier: Southern Biotechnology

CD10, also known as the common acute lymphoblastic leukemia antigen (CALLA), is a type II transmembrane glycoprotein. It is expressed on granulocytes, a subset of B progenitor cells, germinal center cells, and fibroblasts. CD10 is a zinc-binding metalloprotease which is thought to down-regulate cellular responses to peptide hormones.

Supplier: Southern Biotechnology

The CD45 antigen is an essential regulator of leukocyte activation and development and is now known to be a protein tyrosine phosphatase, e.g., CD45 is able to dephosporylate the Src family of protein kinases. This activity is known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling, and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685 and 1820) and as allelic forms CD45.1 (Ly-5.1) and CD45.2 (Ly-5.2). The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. The monoclonal antibody 104 recognizes CD45 (leukocyte common antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).

Supplier: Southern Biotechnology

CD11a, also known as leukocyte function-associated antigen-1 (LFA-1α), represents the 180 kDa integrin αL subunit which combines with the CD18 β2 integrin subunit to form the αLβ2 integrin heterodimer. It is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T cells. CD11a mediates adhesion of lymphocytes to vascular endothelium and is involved in costimulation.