You searched for: Reverse Transcription Reagents

Supplier: Promega Corporation

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in fifteen minutes.

Supplier: New England Biolabs (NEB)

Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity

Supplier: Promega Corporation

GoTaq® RT-qPCR Systems are reagent systems for quantitative analysis of RNA. GoTaq qPCR Master Mix is a ready-to-use, stabilized 2X formulation containing GoTaq(R) Hot Start Polymerase, MgCl2, dNTPs, BRYT Green(R) Dye and a proprietary reaction buffer.

Supplier: Quantabio

UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems that do not require a passive reference dye. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. qScript XLT One-Step RT-qPCR ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Enzo Life Sciences

Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.

Supplier: EMPIRICAL BIOSCIENCE

Inhibitor tolerant reverse transcription PCR/qPCR, reverse transcription PCR/qPCR.

Supplier: Promega Corporation

GoScript Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors.

Supplier: Promega Corporation

The ImProm-II Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification.

Supplier: Promega Corporation

Access RT-PCR System is designed for the reverse transcription and PCR amplification of a specific target RNA from total RNA or mRNA.

Supplier: Lucigen

Lucigen’s NxGen™ M-MulV Reverse Transcriptase is outstanding in cDNA synthesis.

Supplier: AAT Bioquest

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling or PCR.

Supplier: EMPIRICAL BIOSCIENCE

This is a ready-to-use master mix for one-step, reverse-transcription quantitative PCR (RT-qPCR) detection of specific RNA sequences using dye or fluorescent probe-based detection.

Supplier: Promega Corporation

Point mutation of M-MLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.

Supplier: Promega Corporation

Deletion mutation of M-MLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.

Supplier: Promega Corporation

GoTaq RT-qPCR Systems are reagent systems for quantitative analysis of RNA. GoTaq qPCR Master Mix is a ready-to-use, stabilized 2X formulation containing GoTaq(R) Hot Start Polymerase, MgCl2, dNTPs, BRYT Green(R) Dye and a proprietary reaction buffer.

Supplier: Bio-Rad

iScript™ Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR

Supplier: Zymo Research

ZymoScript RT PreMix is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis.

Supplier: New England Biolabs (NEB)

The Monarch spin PCR and DNA cleanup kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription.

Supplier: Zymo Research

The Quick-DNA/RNA MiniPrep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality genomic DNA and total RNA that is ready for any downstream application including reverse transcription, microarray, sequencing, etc.

Supplier: Zymo Research

The RNA Clean & Concentrator™ (RCC™) kits facilitate the efficient removal of RNA polymerases, ligases, and RNA modifying enzymes.

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

Supplier: Promega Corporation

Robust reverse transcriptase. Stable at higher reaction temperatures (37-58 degrees C).

Supplier: EMPIRICAL BIOSCIENCE

Empirical's ActiTemp-50 RTScript™ Reverse Transcriptase is a modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. The enzyme is Warm-Start modified with a proprietary RNA Aptamer.

Supplier: Agilent Technologies

AccuScript High-Fidelity Reverse Transcriptase delivers the highest RT accuracy currently available.

Supplier: Quantabio

Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Supplier: QIAGEN BEVERLY INC MX

The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR.

Supplier: Lucigen

An optimized MMLV reverse transcriptase enzyme for improved production of full-length cDNA from limiting total RNA samples.

Supplier: QIAGEN BEVERLY INC MX

SuperMix provides a sensitive and easy-to-use solution for two-step qRT-PCR

Supplier: Quantabio

qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR