200 Results for: "Promocell"

Supplier: Promocell

The PromoCell Mammary Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the mammary gland

Supplier: Promocell

Supplier: Promocell

Trypsin is the most widely employed enzyme used for cell detachment.

Supplier: Promocell

The PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the cultivation of epithelial cells from large air passages

Supplier: Promocell

Supplier: Promocell

Primary Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are isolated from human pulmonary arteries and stain positive for smooth muscle α-actin

Supplier: Promocell

PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

Supplier: Promocell

Primary Human Tracheal Epithelial Cells (HTEpC) are isolated from the surface epithelium of human trachea and stain positive for cytokeratin.

Supplier: Promocell

Primary Human Renal Epithelial Cells (HREpC) are isolated from the adult kidney and stain positive for cytokeratin.

Supplier: Promocell

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells. Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.

Supplier: Promocell

Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request). Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes.

Supplier: Promocell

Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue

Supplier: Promocell

Primary Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from the arteries of the umbilical cord and stain positive for smooth muscle α-actin

Supplier: Promocell

Supplier: Promocell

Calcium chloride solution allows the adjustment of the optimal amount of calcium required by the cells. Calcium chloride (CaCl₂) solution is prepared as a solution from highly pure tissue culture tested calcium chloride and cell culture-grade water.

Supplier: Promocell

The PromoCell Skeletal Muscle Cell Growth Medium is a low-serum (5% V/V) medium optimized for the expansion of human skeletal muscle cells

Supplier: Promocell

PromoCell Fibroblast Growth Medium 2 is a low-serum (2% V/V) medium developed for adult human fibroblasts

Supplier: Promocell

The Macrophage Detachment Solution DXF is a non-enzymatic reagent especially optimized for the gentle detachment of strongly adherent macrophages.

Supplier: Promocell

This DetachKit is used for gentle and effective detachment of adherent primary human cells in routine subculturing.

Supplier: Promocell

The PromoCell Mononuclear Cell Medium is a very rich short-term maintenance medium which provides utmost cell viability

Supplier: Promocell

Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor. (Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.) The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.

Supplier: Promocell

Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.

Supplier: Promocell

Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin. New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.

Supplier: Promocell

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs. They are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation.

Supplier: Promocell

PromoCell Human CD14+ Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated by positive selection with > 95% purity.

Supplier: Promocell

Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process.

Supplier: Promocell

PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells

Supplier: Promocell

The PromoCell Melanocyte Growth Medium is a serum-free, PMA (Phorbol Myristate Acetate) containing medium optimized for the cultivation of human melanocytes

Supplier: Promocell

Supplier: Promocell

Primary Human Nasal Epithelial Cells (HNEpC) are isolated from normal human nasal mucosa and stain positive for cytokeratin.