2030 Results for: "Moulds+for+Histology"

Supplier: Biotium

Glypican-3 Monoclonal antibody, Clone: GPC3/863, Host: Mouse, Species reactivity: Human, Rat, Isotype: IgG1, kappa, Conjugate: CF405S, Immunogen: Recom full-length human GPC3 protein, Synonyms: DGSX, GPC3, GTR2-2, Application: IF, IHC, Size: 500 uL

Supplier: Biotium

Glypican-3 Monoclonal antibody, Clone: GPC3/863 + 1G12, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF594, Immunogen: Recom fragment containing amino acids 511-580, Synonyms: DGSX, GPC3, GTR2-2, Application: IF, IHC, Size: 500 uL

Supplier: Biotium

Glypican-3 Monoclonal antibody, Clone: GPC3/863 + 1G12, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF647, Immunogen: Recom fragment containing amino acids 511-580, Synonyms: DGSX, GPC3, GTR2-2, Application: IF, IHC, FC, Size: 100uL

Supplier: Biotium

Glypican-3 Monoclonal antibody, Clone: GPC3/863 + 1G12, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF488A, Immunogen: Recom fragment containing amino acids 511-580, Synonyms: DGSX, GPC3, GTR2-2, Application: IF, IHC, Size: 500 uL

Supplier: Electron Microscopy Sciences

Scala C

Supplier: Newcomer Supply

Gram, Gram positive & Gram negative bacteria, artificial control slides contain a section of gram positive and gram negative staining rat lung and a section of human lung nonreactive to organisms.

Supplier: Electron Microscopy Sciences

The 7000smz-2 unit with a Z-axis deflection of Sub-Micron and a blade advance controllable to 10 microns/second

Supplier: Corning

Transwell® cell culture inserts are convenient, easy to use permeable support devices for the study of both anchorage-dependent and anchorage-independent cell lines. They are designed to produce a cell culture environment that closely resembles the in vivo state.

Supplier: Electron Microscopy Sciences

A special mounting media for immunofluorescent staining procedures. It is a water soluble, non-fluorescing medium which is well suited for use when the staining procedure has an aqueous final step. It contains 10% polyvinyl alcohol in phosphate buffered glycerol with 0.1% sodium azide as a preservative.

Supplier: Newcomer Supply

Fungus, GMS, Multi-tissue, artificial control slides contain sections of Aspergillus sp. positive staining rat lung, Candida sp. positive staining rat lung, Histoplasma sp. positive staining animal tissue, and negative staining human lung.

Supplier: MP Biomedicals

Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride.
Phenol Red is used as a pH indicator in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
Store at Room Temperature (15-30 °C)

Supplier: Biotium

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain in terms of convenience and safety. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band.

Supplier: Vector Laboratories

VectaMount™ Permanent Mounting Media protects sections under coverslips and preserves IHC staining patterns.

Supplier: Biotium

ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers.

Supplier: Epredia

The CryoStar™ NX50 Cryostat extends the benefits of the NX series by offering an innovative new look at cryosectioning. The NX series has been designed completely around the user to deliver optimal sectioning quality, unmatched ergonomics, and safety in an energy efficient package. With the NX series, users can deliver high quality results comfortably, safely, and efficiently.

Supplier: Biosensis

Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.

Supplier: Epredia

Improve throughput with the popular Gemini™ AS Automated Slide Stainer, the ultimate multitasking stainer with a footprint small enough to place exactly where it is needed for optimal laboratory workflow.

Supplier: Biosensis

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Supplier: Biosensis

Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C