7792 Results for: "MP Biomedicals"

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Alcohol dehydrogenases are a group of dehydrogenase enzymes that occur in many organisms. It is a metalloenzyme containing four tightly bound zinc atoms per molecule. Each subunit also contains a second zinc atom (conformational zinc), which stabilizes the enzyme’s tertiary structure. Per subunit, there are two distinct active site sulfhydryl groups which can be distinguished on the basis of differential reactivity with iodoacetate and butyl isocyanate. A histidine residue is considered to have an essential role.

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Streptomycin Sulfate is an aminoglucoside antibiotic in which streptidine is linked glycosidically to the disaccharide streptobiosamine.

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From Beef Blood Autoclavable preparation To be used with GC Agar Base.

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Stainless Steel Tubes are ideal for grinding, lysis, and homogenization of the most resistant samples.

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Heat-Shocked Fractionate.

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Actinomycin D is a polypeptide antibiotic first described by Waksman. It is bacteriostatic in nature, especially towards gram negative bacteria, and also has anti-tumor activity.

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Oligomycin is a natural antibiotic isolated from Streptomyces diastatochromogenes, which is a mixture of oligomycins A, B, and C.

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4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.

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(+/-)-Epinephrine is also known as adrenaline and is a hormone as well as a neurotransmitter that is part of a group of monoamines called catecholamines.

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Ferritin, Equine is a shell of 24 protein subunits (apoferritin) and a core Fe 3+ ions with a peptide MW of 440 kDa. Review of the ferritin-like superfamily of iron-storage proteins, virtually ubiquitous in life on earth.
A ubiquitous iron storage protein that plays a key role in iron metabolism. It serves as an intracellular iron reserve (particularly in spleen, liver, intestinal mucosa, and bone marrow) and functions in iron detoxification. Studies have shown that ferritin iron incorporation is mediated by a ferroxidase activity associated with ferritin H subunits and a nucleation center associated with ferritin L subunits. Release of iron from ferritin has an essential role in iron-dependent lipid peroxidation and may contribute to free radical-induced cell damage in vivo. Therefore, by binding iron, ferritin may function as an antioxidant.
Store at +4 °C.

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Alcian Blue 8GX is a cationic dye used to quantitatively determine glycosaminoglycans by forming insoluble complexes in solution.

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Sodium azide used as a preservative for laboratory reagents; a bacteriostatic agent in storage solutions. Inhibits gram negative flora so is therefore included in culture media for the selective isolation of Streptococci and Staphylococci. It has been used to avoid bacterial contamination during RPC-5 column chromatography of DNA fragments.
Catalyst for:
• Oxidative decarboxylation
• Michael addition reactions
Reagent for synthesis of
• Blue fluorescent copolymers
• Metal phosphonates
• Arenes via aminations
Involved in regioselective synthesis of prianosin B
It is also a useful probe reagent, mutagen, and preservative. In hospitals and laboratories, it is a biocide (A biocide is a chemical substance or microorganism which can deter, render harmless, or exert a controlling effect on any harmful organism by chemical or biological means.)
Sodium Azide is an inhibitor of peroxidase, myeloperoxidase, superoxide dismutase, galactose oxidase, catalase, haemoprotein enzymes and O2 evolution in photosynthesis. The mechanism of its inhibition and toxicity may be due to metal ion complexation and displacement from enzyme.

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L-Cystine is a natural proteinogenic amino acid sulfide dimer of L-cysteine.
L-Cystine is a natural proteinogenic amino acid sulfide dimer of L-cysteine. L-cystine is used as a cell culture supplement. L-cystine is also used to characterize and identify specific amino acid transport systems in cells such as system system Xc(─) .
Store at Room Temperature(15-30 °C)

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Chitosan is a cationic marine polymer derived form the shells of crustaceans (e.g., crabs). MP's chitosan is a partially de-acetylated chitin formed by the reaction of chitin with concentrated alkali. Chitin is a high molecular weight linear polymer of anhydro-N-acetyl-glucosamine. Chitin occurs in nature as part of the protective coating of some invertebrates.

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Imidazole is a histamine antagonist.
Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25 °C. It is also a chelator for the binding of various divalent cations.
Solutions can be successfully sterilized by autoclaving, and are stable for at least 2 years at 2-8 °C, protected from light

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MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range.
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa ± 1). Alternatively, volumes of equimolar MES free acid and sodium or potassium MES can be mixed to attain the desired pH.

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Citric acid is a key metabolic intermediate. Citrate is the starting point of the tricarboxylic acid cycle. Its concentration also coordinates several other metabolic pathways. Citric acid can form complexes with various cations, particularly with iron and calcium. In animals, citric acid improves the utilization of nutritional calcium. Citric acid is produced commercially by fermentation of carbohydrates derived from corn starch and from beet molasses.
Citric Acid, Trisodium Salt, Dihydrate is used as a substrate for citrate lyase, a buffer component; an anticoagulant. For anticoagulation use it is typically used at a concentration of approximately 0.129 M (i.e. for 4.5 mL blood use 16.0 mg sodium citrate and 2.1 mg citric acid).
To make a sodium citrate buffer use equimolar concentrations (typically approximately 0.05 M concentration) of citric acid, free acid and sodium citrate. Add equal volumes of each solution and titrate to the desired pH.
Room Temperature

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Storage: Store at Room Temperature (15-30 °C)
Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water), density gradients, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. Sodium chloride is widely used in protein crystallization.

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Glutaraldehyde is a bifunctional protein cross-linking reagent, reacting with NH2 groups to form Schiff's bases.
Sporicidal agent. Glutaraldehyde reacts through cross-linking to impart water resistance to protein and polyhydroxy compounds. It is also a reducing agent for photochemicals. In organic syntheses, the reactive carbonyl groups of glutaraldehyde suggest its use as an intermediate for the production of resins, dyestuffs, and pharmaceuticals. Glutaraldehyde is a disinfectant, which is rapidly effective against vegetative forms of Gram-positive and Gram-negative bacteria. It is also effective against acid-fast bacteria, bacterial spores, some fungi and viruses, including hepatitis B virus and human immunodeficiency virus. It can also be used for stabilization of proteins on agarose beads, activation of polystyrene and glass for immobilization of antibodies and antigens, and coupling peptides onto carrier proteins. Can be used for preparing emulsions for making photographic film. Also for use as an electron microscopy fixative or cell fixation.
Store at +4 °C. Store Under Nitrogen

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Ciprofloxacin is a fluorinated quinolone antibacterial agent which is highly effective against a broad spectrum of bacteria and mycoplasma.
Ciprofloxacin is most commonly used to treat infections of the skin, bone, sinus, lung, abdomen, and bladder. It can also be used to treat some sexually transmitted infections. Additionally, ciprofloxacin can be used after exposure to inhalation anthrax to reduce the chance of developing an infection.
During the proliferation phase of a bacterium a segmental twisting and untwisting of the chromosomes take place. An enzyme called DNA gyrase plays a decisive part in this process. Ciprofloxacin inhibits this DNA gyrase in a way that arrests the bacterial metabolism, since vital information can no longer be read from the bacterial chromosome. Ultimately, it affect DNA replication & cell division.
Store at +4 °C.

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Pure Canavalia ensiformis lectin (Con A) from Jackbean.

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Polyethylene glycol (PEG) is a condensation polymer of ethylene oxide and water. PEGs are susceptible to oxidative degradation in the presence of air. Minimizing the exposure of PEG to elevated temperatures and/or exposure to oxygen, or addition of an antioxidant can limit the amount of degradation. PEGs do not hydrolyze or deteriorate upon storage. PEGs do not support the growth of molds. PEG is incompatible with phenol and may reduce the antimicrobial action of other preservatives.

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N-Dodecane is a liquid alkane hydrocarbon.
N-Dodecane is used as a solvent, distillation chaser, scintillator component. Moreover it is used as a diluent for tributyl phosphate (TBP) in plants reprocessing.

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Uridine-5'-diphosphogalactose Disodium Salt is a donor substrate for galactosyltransferases involved in the addition of galatose (galactosylation) molecules to N-linked and O-linked oligosaccharides and glycerolipids (biosynthesis of galactose-containing oligosaccharides).
UDP-Gal and its analogues are used to study the distribution, specificity and kinetics of galactosyltransferase(s).
Store at -0 °C, desiccate.

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Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy, in protein sequencing, detecting albumin and organic synthesis.
Trichloroacetic acid is used in protein precipitation; has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning. Rats exposed to chronic sublethal amounts of trichloroacetic acid displayed an increase in serum bilirubin with a decrease in hematological proteins and cholesterols along with significant decreases in red blood corpuscles, mean cell volume, mean corpuscular hemoglobin, hemoglobin and hematocrit. In mice the production of liver tumors was associated to Trichloroacetic acid, which is also a known mouse hepatocarinogen. On the guinea pig trichloroacetic acid was observed to be a mild alergen on the skin.
+4 °C

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Lysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.

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Storage: Store at Room Temperature (15-30 °C). Store Desiccated.
L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine. An isoxazoline RGD mimic platelet GPIIb/IIIa antagonist has been prepared by chiral synthesis with L-asparagine as a starting material. L-Asparagine has been utilized in the synthesis of 4-azalysine building blocks for application to combinatorial chemistry.

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Highly purified enzyme maceration from Aspergillus niger used for the isolation of biologically active protoplasts from a wide spectrum of higher plants and tissues.

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The FastCounting™ cell counting kits provide a sensitive and convenient method to determine cell viability in cell proliferation and cytotoxicity assays.

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The FastMitophagy™ detection kit is specifically designed for mitophagy detection in mammalian cells.