7792 Results for: "MP Biomedicals"

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Uncoated areas have a total wettable surface so that liquid spreads evenly over the area of each well. For cell attachment and growth, the slides require only a simple washing in deionized water or detergent followed by sterilization.

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Product is the lyophilized powder of mouse IgG and buffer salts.

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Potassium acetate is used as a buffer in molecular biology research, notably in the isolation of DNA. Potassium acetate may be utilized in the isolation of RNA intermediates and products from in vitro splicing reactions performed with HeLa cell nuclear extract. Potassium acetate has been used to study the interactions between the TATA box binding protein from Pyrococcus woesei and an oligonucleotide with a specific binding site. The use of potassium acetate in the analysis of oligogalacturonic acids by highperformance anion-exchange chromatography has been described.

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Pristane is a natural saturated terpenoid alkane obtained primarily from shark liver oil.
Pristane is used as a biological marker. It known to induce autoimmune diseases in rodents. It is used in research to understand the pathogenesis of rheumatoid arthritis and lupus, as a non-antigenic arthritogenic adjuvant and plasmacytomas.
Store at Room Temperature(15-30 °C)

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Fluorescamine is a non-fluorescent reagent that reacts readily with primary amino groups in amino acids and peptides to form highly fluorescent derivatives, whereas the reagent and its degradation products are non-fluorescent. No significant fluorescence with ammonia. Low background due to hydrolysis.
an plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.

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Glucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation. Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

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Storage: Store at Room Temperature (15-30 °C), dessicate.
Ammonium persulfate is a strong oxidizing agent. It is a radical initiator.
Ammonium persulfate is a polymerization catalyst used with TEMED for polyacrylamide gel formation. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry. Other applications of APS include its use as a reducer and retarder in photography, the manufacture of aniline dyes, electroplating, the decolorizing and deodorizing of oils.

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Storage: Store at room temperature (15-30 °C)
L-Lysine monohydrochloride is widely used as nutritional supplements in food and beverage industries. It can also be used in animal feed as source of L-Lysine. L-Lysine Monohydrochloride can be used in a wide variety of industries including: food production, beverage, pharmaceutical, agriculture/animal feed, and various other industries.
L-Lysine monohydrochloride is a key amino acid in calcium absorption.

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Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

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Storage: Store at Room Temperature (15-30 °C).
Methionine is one of the common sulfur-containing amino acids. Methionine is a common methyl-group donor to various substrates, such as creatine, epinephrine, ergosterol, and choline.

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2-8 CELLsium™ medium is a cytoprotective, protein free, ready-to-use biosolution lacking dimethyl sulfoxide (DMSO) for the short-term storage of cells or tissue.

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FastGro™ is a synthetic, animal-free, chemically defined FBS replacement. It is a fully defined additive suitable for a wide range of cell culture practices. FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived components.

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Paromomycin is a broad spectrum aminoglycoside antibiotic produced by Streptomyces rimosus var

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A synthetic fluoroquinolone antibiotic used to treat urinary tract infections. It is effective against gram-positive and gram-negative bacteria, and its’ mode of action is to inhibit DNA gyrase and topoisomerase IV, thus
preventing cell division.

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Soluble in Water (100 mg/mL, 36%).

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Soluble in ethanol (50 mg/ ml-clear to slightly hazy yellow to yellow-green solution)

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Naloxine is specific and competitive opiate antagonist at mu, kappa, delta and sigma receptors; it blocks the action of sigma-agonists at opioid sites. It displaces morphine and other opiate agonists from the opiate receptor. It is more potent than Nalorphine and is also an effective antagonist for mixed agonist-antagonist drugs such as pentazorcine. Naloxone has no respiratory depressant activity.

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2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

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Tannic Acid is used for enzyme immobilization and protein adsorption

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Solubility (5% aq soln) Clear, Colorless Solution.

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Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae.
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions. Agarose was used to prepare 0.5% agarose gel for Southern blot analysis of the DNA extracted from primate muscles.
The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

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1X Crystalline Lyophilized. Purity is 97 to 99%. Low B12 and Folate. Prepared using Cohn method IV. Source material is non-reactive and is tested for Hbs Ag, Anti-HCV, Anti-HIV-1, Anti-HIV-2, and Syphillis.

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Baker's yeast is of the species Saccharomyces cerevisiae.

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Soluble in water.

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AlbumiNZ™ Low Fatty Acid BSA is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. This albumin has very low endotoxin and low free fatty acids, is tested for the absence of viruses according to 9CFR and has the highest possible biosafety level.

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Methotrexate is a cell cycle arresting agent with varying effects. Methotrexate has been reported to arrest the cell cycle in late G1/S thus leading to the inhibition of the synthesis of DNA, RNA, thymidylates, and proteins. The main mechanism of action is reported to involve the inhibition of enzymes involved in purine metabolism which leads to the accumulation of adenosine or the suppression of intercellular adhesion molecule expression by T cells. Additionally, this compound has been observed to inhibit DHFR.
Methotrexate is used for chemotherapy either alone or in combination with other agents. It is effective for the treatment of a number of cancers including: breast, head and neck, leukemia, lymphoma, lung, osteosarcoma, bladder, and trophoblastic neoplasms. It is also used in treatment of autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It is used to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.

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Energy-rich precursor in the enzymatic biosynthesis of RNA. A potent vasodilator. Induces contractile responses in some tissues.

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Cobalt chloride is utilized in commercial applications such as electroplating, catalyst preparation, painting on glass and porcelain, and in the manufacture of vitamin B12.

Supplier: MP Biomedicals

Propidium iodide is a fuorescent stain for nucleic acids.