7792 Results for: "MP Biomedicals"

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5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. It can be used to label DNA which can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation.

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β-NAD is one of the biologically active forms of nicotinic acid. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.

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Dihydrostreptomycin sulfate is an aminoglycoside antibiotic. It inhibits protein synthesis by interfering with translation.

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Soluble in ethanol (50 mg/mL) giving clear solution.

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Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism.

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Soy hydrolysate is an enzymatic (papain) digest of soybean meal. It is a plant source peptone. Soy hydrolysate is useful in media where high clarity is not required.

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Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic ; but, if needed, it can be dried at 100 °C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

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Vitamin B12, 0.1% in Mannitol is 96% pure vitamin B12 tritrated onto mannitol at 0.1% concentration.

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Soluble in alkaline solutions (1% in 1 N NaOH), glyurol; very slightly to insoluble in water; insoluble in ethanol, ether and alcohol.

Supplier: MP Biomedicals

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.
It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.
Other uses for poly-lysine have been reported as well:
• Conjugation to methotrexate for increased drug transport.
• Microencapsulation of islets.
• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.
• Immobilation of living cells in biocompatible semipermeable microcapsules.
• In the preparation of polycationic beads.
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Poly-D-lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has additionally been shown to eliminate prion proteins from infected cells.

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Adenosine 5'-triphosphate (ATP) disodium salt hydrate is a multifunctional nucleotide, which is essential in a variety of cellular functions.

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Polyvinyl alcohol is the raw material to make polymers. Polyvinyl alcohol is used as a emulsion polymerization aid, as protective colloid, to make polyvinyl acetate dispersions.

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One unit will oxidize one µmole of protocatechuate to 3-carboxy-cis,cis-muconate per minute at pH 7.5 at 37 °C.

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Amino Acid Sequence:Glu-Cys-Gly, Glu-Cys-Gly [disulfide bridge: 2a-2b].

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Miscible in ethanol (0.1 mL/mL, v/v - clear, colorless solution). It can be used at concentration of 0.1% in the mobile phase of an HPLC/LC-MS protocol for the detection of marine bacterioplankton siderophores.

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Spermine is a polyamine involved in cellular metabolism found in all eukaryotic cells. Formed from spermidine through the addition of aminopropyl group to spermidine by spermine synthase. It is found as a polycation at physiological pH.

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Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It is active against gram-positive and gram-negative bacteria. Spectinomycin binds to the 30S subunit of bacterial ribosomes and suppresses protein synthesis.

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Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein.

Supplier: MP Biomedicals

Protamine sulfate is a purified mixture of simple protein principles obtained from the sperm or testes of suitable species of fish, which has the property of neutralizing heparin. Because of having much basic amino acids (mostly arginine) protamine contains far greater nitrogen than other proteins. Its molecular weight is relatively small. Histone and other basic proteins in the testes of unmatured fishes convert into protamine along with the growth of the fishes. In the testes protamine takes the form of nucleoprotamine linked with DNA.

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Salicin is an alcoholic β-glucoside derived from willow bark that produces anti-inflammatory (including analgesic and antipyretic) effects very similar to that of aspirin Salicin is a substrate for β-glucosidase.

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Sodium selenite is commonly used as a source of selenium in biological research, where selenium is an essential trace element that is normally provided by serum. Selenium is present in selenoproteins such as glutathione peroxidase and thioredoxin reductase, which contain the selenium analog of cysteine, selenocysteine. In particular, glutathione peroxidase has a role in detoxification in vivo as a scavenger of peroxides. Sodium selenite is included in various medium supplements for use in cell culture. Sodium selenite has been utilized in studies of cell proliferation and cancer research. It has been used to alter gene expression in HepG2 cells as analyzed by cDNA microarrays. Sodium selenite can inhibit zinc finger protein/DNA interactions. Sodium selenite has been shown to alter mitochondrial membrane potentials and thus potentially contribute to apoptosis.

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Soluble in water. Reacts slowly with ethanol. Aqueous solutions are most stable in the presence of small amounts of NaOH (0.2% for a nearly saturated solution containing 44% sodium borohydride) and can be kept for several days. Solutions are rapidly decomposed by boiling.

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Triethanolamine is a zwitterionic buffer.

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Fine whole wheat flour, stone ground is the food prepared in the grinding and bolting of cleaned hard wheat.

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Peptone is used in culture media in bacteriology, the growth requirements of most non fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.

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A metabolite with a broad antibiotic spectrum

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Yeast Hydrolysate Enzymatic from Brewers Yeast

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Bromothymol blue sodium salt, powder

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Brilliant Green is one of the triarylmethane dyes. It is closely related to malachite green.

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Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding.