Sulfo-NHS-LC-LC-Biotin, EZ-Link™, Pierce™
Supplier: Invitrogen
Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin enables simple and efficient biotin labeling of antibodies, proteins, and any other primary amine–containing macromolecules. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.
Expand 2 Items
DSG (Disuccinimidyl glutarate), Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.
Expand 2 Items
Pierce™ Agarose II, Low Melting Point, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Agaroses are highly purified and carefully blended formulations of low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.
Expand 1 Items
Pierce™ Reacti-Bind™ Glutathione Coated 96-Well Plates, Thermo Scientific
Supplier: Invitrogen
Pierce™ 96-well glutathione coated plates are PS plates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins
Expand 3 Items
Pierce™ Glutathione Spin Columns, Thermo Scientific
Supplier: Invitrogen
This Thermo Scientific Pierce GST Spin Purification Kit contains Pierce Glutathione Spin Columns and buffer components to provide a fast, easy-to-use format for purifying GST-fusion proteins from cellular lysates.
Expand 3 Items
Pierce™ Recombinant Protein A Plus Agarose, Thermo Scientific
Supplier: Invitrogen
Pierce™ Recombinant Protein A agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilized for use in IgG purification applications where the native ligand has undesirable effects.
Expand 2 Items
Pierce™ Streptavidin, Hydrazide-Activated, Thermo Scientific
Supplier: Invitrogen
Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.
Expand 1 Items
Pierce™ AminoLink™ Plus Immobilization Kit, 2 ml, Thermo Scientific
Supplier: Invitrogen
The AminoLink Plus Immobilization Kit contains AminoLink Plus Coupling Resin and additional reagents needed to perform 5 protein immobilizations, with expected yields of 2 to 40 mg protein per column.
Expand 2 Items
Pierce™ Coomassie Plus™ (Bradford) Protein Assay Kit, Thermo Scientific
Supplier: Invitrogen
The Pierce™ Coomassie Plus™ Protein Assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.
Expand 1 Items
Pierce™ UltraLink™ Hydrazide Resin Affinity Chromatography Resin, Thermo Scientific
Supplier: Invitrogen
UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.
Expand 1 Items
Pierce™ Streptavidin Plus UltraLink™ Resin, Thermo Scientific
Supplier: Invitrogen
Pierce™ Streptavidin Plus UltraLink™ resin consists of purified recombinant streptavidin that has been covalently immobilized onto a beaded acrylamide support
Expand 2 Items
Accessories for Pierce™ AminoLink™ Immobilization Kit, Thermo Scientific
Supplier: Invitrogen
Group: AminoLink Kit and AminoLink Coupling Gel. Links with primary amines on proteins, peptides, antigens or antibodies. Features/Benefits:. AminoLink Coupling Gel is a 4% cross-linked beaded agarose support, activated to form aldehyde functional groups.
Expand 1 Items
Pepstatin A Protease Inhibitor
Supplier: Invitrogen
Pepstatin A Protease Inhibitor reversably inhibits aspartic acid proteases.
Expand 1 Items
Pierce™ Streptavidin Magnetic Beads, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Streptavidin Magnetic Beads accelerate throughput for automated magnetic purification of biotinylated molecules.
Expand 2 Items
Halt™ Phosphatase Inhibitor Cocktail, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.
Expand 4 Items
Imject™ Pierce™ Maleimide-Activated Carrier Proteins, Thermo Scientific
Supplier: Invitrogen
Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten
Expand 4 Items
Pierce™ Imject™ Bovine Serum Albumin (BSA)
Supplier: Invitrogen
Thermo Scientific Imject BSA in PBS is purified bovine serum albumin carrier protein that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.
Expand 1 Items
E-64
Supplier: Invitrogen
E-64 Protease Inhibitor irreversably inhibits cysteine proteases.
Expand 1 Items
ELISA Wash Buffer (30X), Thermo Scientific
Supplier: Invitrogen
ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.
Expand 1 Items
Pierce Zeba™ Desalt Spin Columns, Thermo Scientific
Supplier: Invitrogen
The Zeba™ spin desalting columns are made of low protein binding polypropylene and are compatible with a wide range of standard laboratory instruments and consumables.
Expand 15 Items
Pierce™ HisPur™ Cobalt Spin Filter Plates, 96 Well, Thermo Scientific
Supplier: Invitrogen
HisPur™ Cobalt spin plates are convenient, ready-to-use, pre-dispensed filter plates for the efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells
Expand 1 Items
Pierce™ Chemiluminescent Nucleic Acid Detection Module, Thermo Scientific
Supplier: Invitrogen
A complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs), electrophoretic mobility shift assays (EMSAs or gel shifts).
Expand 1 Items
Pierce™ Affinity Chromatography Cartridges, Protein G, Thermo Scientific
Supplier: Invitrogen
These cartridges are convenient, ready-to-use prepacked devices for isolation and purification of mammalian polyclonal and monoclonal antibodies from serum, ascites and cell culture supernatants.
Expand 2 Items
Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
Expand 1 Items
Pierce™ Protein A, Biotin
Supplier: Invitrogen
Thermo Scientific Pierce Protein A to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
Expand 1 Items
Pierce™ HRP Substrates, 1-Step™ Turbo TMB-ELISA, Thermo Scientific
Supplier: Invitrogen
TMB (3,3´,5,5´ tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP).
Expand 1 Items
Pierce™ UltraLink™ Iodoacetyl Resin, Affinity Purification Resins, Thermo Scientific
Supplier: Invitrogen
UltraLink™ Iodoacetyl Resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblization of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.
Expand 1 Items
ELISA Blocker Blocking Buffer, Thermo Scientific, Thermo Scientific
Supplier: Invitrogen
ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.
Expand 1 Items
Pierce™ Melon™ Gel Purification Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Melon Gel Purification Buffer, formulation: proprietary buffer dry-blend, sufficient for preparing 200 mL of 100X buffer.
Expand 1 Items
Pierce™ Nucleic Acid Detection Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
Nucleic Acid Detection Blocking Buffer is intended for use with the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module Kit, of which it is also a component