"GeCKO Libraries"

Supplier: Vector Biolabs

AAV2/1 expression LacZ (b-Gal).

Supplier: Vector Biolabs

The protein encoded by this gene is a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR delta and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.12.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: Promega Corporation

These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

Supplier: Vector Biolabs

Three cell membrane receptor tyrosine kinases Flt-1 (also designated VEGF-R1), Flk-1 (also designated VEGF-R2 or KDR) and Flt-4 (putatively involved in the growth of endothelial cells) are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domains. Two members of this receptor class, Flt-1 and Flk-1, represent high affinity receptors for vascular endothelial growth factor (VEGF). On the basis of structural similarity to Flt-1 and Flk-1, Flt-4 may represent a third receptor for either VEGF or a VEGF-related ligand. Flt-3/ Flk-2 and its human homolog, STK-1, are expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues.

Supplier: Vector Biolabs

Members of the IKB family can be divided into four groups including IkB-Alpha (including proteins previously referred to as pp40 and MAD-3), IKB-beta, IKB-gamma (a 70 kDa protein identical in sequence to the C-terminal domain of the p105 precursor of NFkB p50) and IkB-epsilon. Members of the IKB family exhibit differential inhibition of the DNA binding activity of various NFkB homodimeric and heterodimeric complexes. A functionally related protein, Bcl-3, is overexpressed in chronic lymphocytic leukemia.

Supplier: Vector Biolabs

PUMA (p53 up-regulated modulator of apoptosis) is a BH3-only protein. Each of the BH3-only group of proteins contains an amphipathic Bcl-2 homology (BH) domain. These proteins act as sensors of discrete apoptotic stimuli, antagonizing or activating the function of multidomain Bcl-2 family proteins by interacting with them. This in turn triggers a cascade of downstream events, such as the collapse of mitochondrial membrane potential, release of the apoptogenic mitochondrial proteins cytochrome c and SMAC, and activation of caspases. PUMA plays a vital role in mediating the apoptotic responses to p53. It can be activated by p53 directly through p53-responsive elements in its promoter region. The PUMA protein interacts with Bcl-XL and promotes mitochondrial translocation and multimerization of Bax. This adenovirus expresses human PUMA gene with a 15-aa deletion of the BH3 domain.

Supplier: Vector Biolabs

Protein phosphatases play critical roles in the regulation of signal transduction pathways. The family can be separated into three general categories, which are distinguished on the basis of substrate specificity. The first and largest category, termed protein tyrosine phosphatases or PTPs, includes transmembrane proteins, membrane associated proteins and proteins that localize to nuclei. Transmembrane PTPs, such as PTP precursors alpha, beta, gamma, epsilon, LAR, HePTP, RPTP-alpha, PTPRN2 and CD45 are involved in homophilic binding and facilitate cell-cell communication. Membrane associated PTPs include the following proteins SH-PTP1 (PTP1C, HCP, SHP), SH-PTP2 (Syp, PTP1D), PTP-H1 and PTP1B. Fap-1 (also designated PTP-BAS) contains an ezrin-like membrane binding domain and through its association with FAS presumably regulates FAS signaling. PTPs, such as KAP (also designated Cdi1), function to regulate the activity of proteins in the nuclei of cells. The second category of protein phosphatases dephosphorylate proteins on phosphoserine and phosphothreonine residues, whereas the third category of protein phosphatases exhibit dual specificities and can dephosphorylate proteins on phosphotyrosine and phosphoserine/phosphothreonine residues.

Supplier: Vector Biolabs

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest.

Supplier: Vector Biolabs

Differentiation of myogenic cells is regulated by multiple positive and negative regulators. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5, Myf-6 (also designated MRF-4 or herculin) and musculin (also designated MyoR). Members of this group of transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, ITF2 and HEB. The E2A gene encodes at lest two different proteins, E12 and E47, as a result of alternative splicing. The myocyte enhancing factor-2 (MEF-2) family of transcription factors includes four splice variants A, B, C and D which encode muscle-specific DNA binding proteins that recognize an A/T rich sequence [CTA (A/T)4 TAG] localized in the control regions of numerous muscle-specific genes.

Supplier: Vector Biolabs

Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP [inhibitor of apoptosis protein] binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and removing their inhibitory activity. In healthy cells, Smac resides in the mitochondria, but when cells undergo apoptosis, Smac is released into the cytosol.

Supplier: Vector Biolabs

c-Myc is a very strong proto-oncogene and it is very often found to be upregulated in many types of cancers. It is a mammalian transcription factor belonging to the bHLH (basic Helix Loop Helix)-Leucine Zipper family. The Myc family of cellular oncogenes, which includes c-Myc, N-Myc, L-Myc, S-Myc and B-Myc, represents nuclear transcription factors that play a significant role in cellular proliferation, differentiation, apoptosis and transformation. Members of this gene family are activated upon various mitogenic signals such as Wnt, Shh and EGF. They form heteromeric complexes with other interacting partners, such as members of the Max and Mad families, to activate transcription. Alternatively, c-Myc can also inhibit the DNA binding protein Miz-1 to repress a second set of target genes. Mnt and Mlx regulate Myc activity by forming heterodimers with Max or Mad, respectively, to suppress Myc-induced transcriptional activation. This adenovirus contain human c-Myc. It was constructed using AdEasy system.

Supplier: Vector Biolabs

Oct-3/4 is one of the family of octamer ("Oct") transcription factors, and plays a crucial role in maintaining pluripotency. The absence of Oct-3/4 in Oct-3/4+ cells, such as blastomeres and embryonic stem cells, leads to spontaneous trophoblast differentiation, and presence of Oct-3/4 thus gives rise to the pluripotency and differentiation potential of embryonic stem cells. Various other genes in the "Oct" family, including Oct-3/4's close relatives, Oct1 and Oct6, fail to elicit induction, thus demonstrating the exclusiveness of Oct-3/4 to the induction process.Co-expression of Oct4, Sox2, KLF4 and c-Myc (Yamanaka set) in somatic cell will lead to iPSC reprogramming. Using adenovirus to deliver these transcription factors has 2 main advantages - it does not incorporate into the targeted host and therefore avoids the potential for insertional mutagenesis, and the expression is transient for a brief amount of time in order for effective reprogramming to take place. This adenovirus expresses human Oct4, and it can be used in combination with other iPSC factor for the induction of iPSC reprogramming.

Supplier: Vector Biolabs

Fas Ligand Adenovirus (Ad-CMV-FasL)

Supplier: Vector Biolabs

T cell factor-4 (Tcf-4) plays an important role in development and carcinogenesis. It directly binds to cytosolic β-catenin and facilitate its translocation to the nucleus. In the nucleus, these complexes induce expression various target genes. The adenovirus expresses a dominant negative TCF4.

Supplier: Vector Biolabs

High mobility group (HMG) proteins are non-histone DNA-binding proteins that induce alterations in the DNA architecture, including DNA bending and unwinding of the helix. Although HMG proteins may bind to single-stranded DNA, conformational changes are only induced in double-stranded DNA. HMG proteins synergize with Oct-2, members of the NFkB family, ATF-2, c-Jun and members of the steroid class of receptors to activate transcription.