176307 Results for: "Di-boc-3,4-diaminobenzoic+acid"

Supplier: Bioss

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Supplier: Bioss

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Supplier: Eagle Biosciences

Mouse Rat Urocortin 1 ELISA determines the concentration of urocortin 1 in mouse & rat plasma,serum.

Supplier: Globe Scientific

Globe Glass™ volumetric flasks are calibrated to meet ASTM tolerance specifications for precise measurement in solution preparation and dilution. A diamond etched graduation line indicates the calibrated capacity of each measuring flask. Wide mouth options are available for easier filling and mixing. Globe Glass™ volumetric flasks are offered in sizes ranging from 1 to 2000 ml, and with Class A or Class B calibration tolerances. Class A volumetric flasks include a batch calibration certificate.

Supplier: Promega Corporation

The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays

Supplier: Prosci

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Supplier: Promega Corporation

The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays

Supplier: DRG International

An enzyme immunoassay for the qualitative and semiquantitative determination of IgG-class antibodies to Chlamydia trachomatis in serum and plasma.

Supplier: Bioss

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Supplier: Bioss

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Supplier: Bioss

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Supplier: Promega Corporation

The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays

Supplier: Cytiva

AcroPrep Advance 96-well filter plates are chemically compatible with harsh organic and / or aqueous solvents and contain inert materials of construction to provide a stable platform and ensure complete recovery of samples and low non-specific binding. The water wettable Polytetrafluoroethylene (wwPTFE) is a universally chemically compatible membrane and offers fast flow rates, low protein binding, and low levels of UV-absorbing extractables. These plates are suitable for applications such as metabolic studies, molecular or drug synthesis reactions, sample prep using solvents, and aggressive filtration applications.

Supplier: Corning

Corning® Synthegel™ 3D matrices are chemically defined peptides that self assemble into nanofibers which crosslink to form tuneable hydrogels. These hydrogels provide a robust and reproducible microenvironment for 3D culture of physiological spheroids and hiPS cells. The Synthegel™ matrix kits provide negligible variability and a more controlled substrate for in vivo-like 3D cell culture. Such a platform is needed for cancer research, stem cell research, and drug screening in a 3D format.

Supplier: MP Biomedicals

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: Promega Corporation

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Supplier: Prosci

RAE1 is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly (A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Supplier: Rockland Immunochemical

K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54% identity with TASK-1, but less than 30% identity with TASK-2 and other tandem pore K+ channels. Anti-TASK1 Potassium Channel Antibody is ideal for research in Neuroscience, particularly studies concerning the dopaminergic neuron and melatonin signaling.

Supplier: Cytiva

The Acroprep 24-well filter plate utilizes Pall’s proprietary high-performance multi-layer filter media and membranes to offer time savings, strong performance claims and streamlined workflow improvements in a 24-well plate format.

Supplier: Rockland Immunochemical

Humanized Recombinant Anti-VEGFA ScFV fragment Antibody has been tested for use in Flow Cytometry, Western Blot, and ELISA. This antibody recognizes structured VEGF-A and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

Supplier: DRG International

An enzyme immunoassay for the qualitative and semiquantitative determination of IgA-class antibodies to Chlamydia trachomatis in serum and plasma.

Supplier: DRG International

An enzyme immunoassay for the qualitative and semiquantitative determination of IgM-class antibodies to Chlamydia trachomatis in serum and plasma.

Supplier: Promega Corporation

The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays

Supplier: Molecular Devices

The GenePix® 4300A and GenePix® 4400A Scanners from Molecular Devices offer maximum imaging quality with optimal resolution in highly configurable platforms.

Supplier: Labconco

These stations provide user protection by keeping powders and particulates contained during weighing procedures

Supplier: WORLD PRECISION INSTRUMENTS LLC

The properties of this bio-compatible surgical adhesive make it exceptionally useful for neuroscience applications, peripheral nerve studies and similar biomedical applications. These  silicone elastomers also eliminate the mess and time involved in pre-mixing other commonly used formulations (such as Wacker SilGel^®and Sylgard^®). Each silicone elastomer is packaged in a double barrel syringe and is automatically mixed when pressed out of the mixer tip provided.

Supplier: Agilent Technologies

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

Supplier: Cytiva

The key to reproducible electrophoresis results is temperature control. Gels cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels. SE 600 Ruby ensures uniform heat in gels because, the gels are immersed in the lower buffer for uniform heat transfer and a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature. The ergonomic SE 600 Ruby provides two built-in side handles, which facilitates carrying and handling. Run up to four gels (up to 112 samples) in a single run. Divider plates convert single-gel sandwiches into two-gel club sandwiches, which seal and fit into the casting stand like single gels. If gels longer than 16-cm are required, Cytiva offers the SE 660, which runs gels up to 24 cm. Except for gel lengt, the SE 660 has identical features to those of SE 600 Ruby.

Supplier: Ohaus

Ranger® 4000 Compact Industrial Counting Scales offer the best value for durable industrial weighing and counting applications. The largest, white LED display on any scale in its class, combined with checkweighing LEDs and audible indicators, make the Ranger® 4000 highly functional in loud and dim industrial settings. The high resolution Ranger® Count 4000 has a 1:30,000 display resolution and 1:1,500,000 internal counting resolution. It also features advanced auto-optimization software which recalculates the average piece weight as the overall weight increases, ensuring accuracy and minimizing counting errors.