"Cube Biotech"

Supplier: Cube Biotech

PureCube Ni-NTA MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: Cube Biotech

The PureCube Ni-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Ni-NTA resin.

Supplier: Cube Biotech

This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein.

Supplier: Cube Biotech

PureCube Ni-INDIGO magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: Cube Biotech

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria.

Supplier: Cube Biotech

The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

PureCube Maleimide Agarose resin is your best option to couple biomolecules that carry thiol (SH) groups.

Supplier: Cube Biotech

Our PureCube HiCap StrepTactin® agarose XL resin beads consist of 6% cross-linked agarose coupled to the StrepTactin protein.

Supplier: Cube Biotech

PureCube amine activated MagBeads are your best option to couple biomolecules that carry free-standing carboxy (COOH) groups.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection.

Supplier: Cube Biotech

PureCube StrepTactin® MagBeads XL is a suspension of 6% cross-linked magnetic agarose beads coupled to the StrepTactin protein.