"Corning"

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

Supplier: Corning

This is a combination of penicillin, streptomycin and Amphotericin formulated to contain 10000 units/ml penicillin G, 10 mg/ml streptomycin sulfate and 25 µg/ml amphotericin B.

Supplier: Corning

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of trypsin.

Supplier: Corning

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

Supplier: Corning

L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment

Supplier: Corning

100 mM Solution with 8.5 g/L NaCl Sodium pyruvate is often used as a component in culture broth and media as an energy source for propagating cells. This reagent is also used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid.

Supplier: Corning

Hygromycin B, produced by Streptomyces hygroscopicus, is used as a selection agent and inhibits protein synthesis in cells not carrying hygromycin phosphotransferase (HPH)

Supplier: Corning

This antimycotic interferes with fungal membrane permeability by forming channels in cell membranes, causing small molecules to leak from the cell. It is recommended for use in cell culture applications at 10 ml/L and is stable at 37 ºC for 3 days.

Supplier: Corning

Miller's LB broth is based on the original Lysogeny Broth (LB) media formulation developed by Luria and Bertani, which was designed for the rapid propagation of recombinant E. coli strains.

Supplier: Corning

Terrific Broth is a medium for the propagation and maintenance of recombinant E.coli.

Supplier: Corning

Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification

Supplier: Corning

TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis