Cal-520® NHS Ester
Supplier: AAT BIOQUEST INC
Calcium measurement is critical for numerous biological investigations.
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5-Carboxyfluorescein-N-succinimidyl ester (5-FAM SE)
Supplier: Adipogen
Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 5-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Used in fluorescent small molecule preparations. Spectral data: lambdaex 494 nm| lambdaem 520 nm (Buffer pH 9.0).
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EQ1 NHS ester amine-reactive quencher dye
Supplier: Enzo Life Sciences
Amine-reactive quencher dye has a broad visible absorption but no detectable fluorescence emission in fluorescence resonance energy transfer (FRET) applications.
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6-Carboxyrhodamine 6G succinimidyl ester (6-CR6G SE) ≥95% (by TLC) amine-reactive fluorescent dye
Supplier: Biotium
Amine-reactive form of 6-carboxyrhodamine 6G single isomer.