"Buffer Solution - Blue"

Supplier: MilliporeSigma

Buffer solution (boric acid/potassium chloride/sodium hydroxide) colour coded: blue, traceable to NIST and PTB pH 10.00 (25°C) Certipur®.

Supplier: Honeywell Research Chemicals

These buffer solutions are color-coded for easy identification and the pH 4 and 7 version have fungicide additive.

Supplier: MERIDIAN LIFE SCIENCE INC

DNA loading buffer blue (5X) is a convenient ready-to-use solution to prepare samples for DNA electrophoresis.

Supplier: Electron Microscopy Sciences

pH 6.5 is typically used with Wrights stain and potassium ferricyanide solution for Prussian Blue staining of non hemoglobin iron by method of Sundberg and Broman (1955). pH 7.0 buffer is used with Giemsa stain.

Supplier: Biolegend

CD3, Monoclonal Antibody, Clone: SK7, Host: Mouse, Species Reactivity: Human, Cross-Reactivity: Chimpanzee, Isotype: IgG1, Conjugate: Spark Blue 550, Other Names: T3, CD3E, Formulation: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide and 0.2% (w/v) BSA, Size: 100 Tests

Supplier: Antyila Scientific

Fresh pH calibration solution anytime and anywhere.

Supplier: Antyila Scientific

Ideal for laboratory and field analysis

Supplier: Aladdin Scientific

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.TMB-D Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.The substrate does not contain NMP (1-methyl2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.Product Characteristics TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.TMB-D Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site. The substrate does not contain NMP (1-methyl-2- pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use.Composition and Properties Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.Working Procedure The following procedure is applicable to nitrocellulose membranes. The procedure must be optimized for other membranes.1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature, in the dark, before use. 2.After the last incubation with HRP-labelled Streptavidin or HRP-labelled secondary antibody it is recommended to wash the membrane in a 0.1 M Tris buffer pH 7.4.3.Shake off the excess buffer and incubate the membrane in the TMB-D Blotting solution for 10 minutes. 4.Wash the membrane in distilled water and allow it to dry. 5.The site of positive reaction will appear light blue with no or very little background staining.Tips and Tricks • The membrane can be blocked with Kementec’s Synthetic Blocking Buffer for Blotting, (cat. no. S494457). • For long-term preservation of the results, the membranes must be stored in the dark.Handling and Storage • Store solution at 2-8⁰C in the dark. • Avoid exposure to light, heat and contamination with metal ions or peroxidase. • Re-dispense only into bottles made of High-Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.

Supplier: Aladdin Scientific

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.TMB Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.The substrate does not contain NMP (1-methyl-2pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.Product Characteristics TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.TMB Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.The substrate does not contain NMP (1-methyl-2- pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use. Composition and Properties Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added. Working Procedure The following procedure is applicable to nitrocellulose membranes. The procedure must be optimized for other membranes.1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use. 2.In order to prevent the blotted membrane from fading, we recommend the use of 0.1M K₂HPO₄, pH 7.2 in coating.3.After the last incubation with HRP-labelled Streptavidin or HRP-labelled secondary antibody, it is recommended to wash the membrane in a 0.1 M Tris buffer pH 7.4. 4.Shake off the excess buffer and incubate the membrane in the TMB Blotting solution for 10 minutes. 5.Wash the membrane in distilled water and allow it to dry. 6.The site of positive reaction will appear light blue with no or very little background staining. Tips and Tricks. The membrane can be blocked, prior to applying the substrate, with Kementec’s Synthetic Blocking Buffer for Blotting (cat. no. S494457). For long-term preservation of the results, the membranes must be stored in the dark.Handling and Storage. Store solution at 2 to 8 °C in the dark. Avoid exposure to light, heat and contamination with metal ions or peroxidase. Re-dispense only into bottles made of High-Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.

Supplier: CANCER DIAGNOSTICS, INC.

The Endure Staining System is designed to produce precise, consistent H&E staining.

Supplier: OZ Biosciences

OZ Biosciences X-Gal Staining Kit allows to visualize -gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates

Supplier: Rockland Immunochemical

2X SDS-PAGE Sample Buffer

Supplier: BDH

BDH pH buffers are available as either colorless or colored solutions.

Supplier: Aladdin Scientific

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.TMB EASE characteristics are:1:1 replacement of carcinogenic 2mg OPD  Aqueous based – no organic solvents (REACH Annex XVII compliant)Low lot-to-lot variationProduct CharacteristicsTMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.TMB EASE is a ready-to-use and stable aqueous formulation. This substrate gives a slow assay reaction and is highly suitable for the direct switch from OPD (ortho-phenylenediamine dichloride) based assays. It does not contain NMP (1-methyl-2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use.Composition and PropertiesReady-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.Working Procedure1. The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use.2. A recommended volume of 100 µl substrate solution per microtiter well is added. Time of development is typically 5-30 minutes. Keep the plate in the dark during incubation.3. In kinetic assays, the blue color is read directly at 650 nm. For endpoint assays 100 µl (equal volumes) of 0.20 M sulphuric acid is added and the absorbance is read at 450/620 nm. The yellow solution is stable for at least one hour.Tips and Tricks • If reduced intensity is required, it is recommended to shorten the time of development or to dilute the conjugate/antibody in the immunoassay further. • Dilution of the substrate is not recommended.• In case of incidents of precipitation with very high OD-values, we can recommend the use of the TMB X-tnd (cat. no. T494100). Handling and Storage • Store solution at 2-8 °C in the dark. • Avoid exposure to light, heat and contamination with metal ions or peroxidase. • Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. • Dispensing guidelines are available upon request.

Supplier: G-Biosciences

TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.

Supplier: Biolegend

Pacific Blue™ anti-human CD3 [UCHT1]; Isotype: Mouse IgG1, κ; Reactivity: Human, Cross-Reactivity: Chimpanzee; Apps: FC; Size: 500 tests