263 Results for: "Biosensis"

Supplier: Biosensis

The Biosensis proBDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in human serum and plasma (citrate and EDTA), cell culture supernatants, mouse and rat cell lysates and rat brain only if used as directed.

Supplier: Biosensis

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family. Antibody reacts with human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.

Supplier: Biosensis

mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).

Supplier: Biosensis

Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

Supplier: Biosensis

Tyrosine Kinase Receptor C, TrkC, 397-408, Monoclonal Antibody, Host: mouse, Clone: BS337, Species: Human, Cross-reactivity: Mouse and rat TrkC, Purity: Protein G purified, Isotype: IgG2b, kappa, Application: Flow Cytometry, Tested on human and rodent cell lines, Storage: 2-8C, Size: 100ug

Supplier: Biosensis

Beta casein protein polyclonal antibody, Host: Rabbit, Reacts with Bovine, Immunogen: Bovine beta-casein peptides, Detects both A1 and A2 beta-casein isoforms, Purity: Affinity purified, and absorbed, Form: Liquid i

Supplier: Biosensis

Calretinin Monoclonal antibody, Clone: 3G9, Host: mouse, Reactivity: human, cow, rat, mouse, Isotype: IgG1, Immunogen: Full-length recombinant human protein, Synonyms: CR; 29 kDa calbindin, Applications: WB, IHC, S

Supplier: Biosensis

IgG1 isotype control antibody. This control IgG has no known binding ability. No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.

Supplier: Biosensis

Mouse anti-green fluorescent protein (GFP) monoclonal antibody (unconjugated), suitable for WB, ICC.

Supplier: Biosensis

Chicken anti-green fluorescent protein (GFP) polyclonal antibody (unconjugated), suitable for WB, ICC.

Supplier: Biosensis

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Supplier: Biosensis

The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (Aβ) associated with the pathology characteristic of Alzheimer’s disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for Aβ have been shown to actually detect intraneuronal APP and not Aβ exclusively. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide. MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ40 and Aβ42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, distinct from Aβ associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.

Supplier: Biosensis

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. The Spectrins form into dimers and further into tetramers of alpha and beta subunits (Ref: Entrez Gene). The alpha-II subunit is widely expressed in tissues but, in the nervous system, is found predominantly in neurons.

Supplier: Biosensis

Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.

Supplier: Biosensis

Recognizes native and denatured forms of Cyan Fluorescent Protein (CFP) and its variants: Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).

Supplier: Biosensis

Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Supplier: Biosensis

Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.

Supplier: Biosensis

The Biosensis NGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of human NGF in less than 3 hours in cell culture supernatants, serum, plasma (citrate) and brain extracts only if used as directed.

Supplier: Biosensis

The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular mouse serum samples.

Supplier: Biosensis

The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Supplier: Biosensis

The biosensis® Multi-Neurotrophin RapidTM Screening ELISA kit has been designed to allow rapid screening and quantification of mouse NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

Supplier: Biosensis

The Biosensis NGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed.

Supplier: Biosensis

TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com). Reacts with human TrkA. Known to cross-react with TrkA from rat and mouse. Expected to cross-react with other mammalian species based on peptide antigen sequence similarity.

Supplier: Biosensis

P75NTR Monoclonal Antibody, Clone: ME20.4, Host: Mouse, Species Reactivity: human, cat, dog, pig, Purity: Protein G purified, IgG was labelled with ATTO 488 and free dye removed by filtration, Other Names: Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD, Size: 50ug

Supplier: Biosensis

MeCP2 antibody, Host: Rabbit, Reacts with human, mouse, rat, Isotype: IgG1, Immunogen: synthetic peptide (REEPVDSRTPVTERVS, aa 471-486) of C-terminus of human protein, Synonyms: MeCp-2 protein; Store lyophilised a

Supplier: Biosensis

MeCP2 Monoclonal antibody, Clone: 4F11, Host: Mouse, Reacts with human, mouse, rat, Isotype: IgG1, Immunogen: Full-length recombinant human protein, Synonyms: MeCp-2 protein; MeCp2, Store lyophilised antibody at 2

Supplier: Biosensis

Nestin antibody, Host: Rabbit, Reacts with human, Purity: whole serum, Antigen: Part of recombinant human protein (amino acids 315-630), Applications: WB, ICC, Storage: Store lyophilised antibody at 2-8C, Size: 10

Supplier: Biosensis

Laminin-111 antibody, Host: Rabbit, Reacts with mouse and rat, Purity: affinity purified, Antigen: Laminin-111 isolated from mouse EHS cells, Store lyophilised antibody at 2-8C, Applications: WB, ICC, IHC, Size: 10

Supplier: Biosensis

Normal serum Antibody, Host: sheep(non-immunized animal), Purity: Whole serum, Form: Lyophilized, without preservatives, Application: Use as negative control for IgG-purified sheep primary antibodies, Storage: lyop

Supplier: Biosensis

Parvalbumin antibody, Host: Chicken, Reacts with human, rat, Mouse, Purity: IgY fraction, Antigen: Full-length recombinant human protein, Applications: WB, IHC, Storage: Store lyophilised antibody at 2-8C, Size: 5