8143 Results for: "Bachem Americas"

Supplier: Bachem Americas

Mca-PLGL-Dpa-AR-amide is a very sensitive FRET substrate for continuous assays and in situ determination of the matrix metalloproteinase activity. Cleavage at the Gly-Leu bond separates the highly fluorescent Mca group from the efficient 2,4-dinitrophenyl quencher, resulting in an increase in fluorescence intensity. The kcat/Km values for the punctuated metalloproteinase (MMP-7) and for gelatinase (MMP-2), e.g., are 1.7·10⁵ and 6.3·10⁵ M⁻¹s⁻¹, respectively.

Supplier: Bachem Americas

This internally quenched fluorogenic peptide substrate contains anthranilic acid as fluorescent donor and m-nitro-tyrosine as acceptor (quencher). The sequence Abz-RVKRGLAY(NO₂)E is based on the sequence of hemagglutinin. The FRET substrate is efficiently cleaved by furin, a subtilisin-like eukaryotic serine endoprotease with Km = 3.8 µM and kcat = 29.3 s⁻¹ (kcat/Km = 7'710'000 M⁻¹s⁻¹). Its kcat/Km value is over 2000-fold higher than that of the commonly used substrate Boc-Arg-Val-Arg-Arg-AMC (I-1645). FRET Substrate for the basic residue-specific yeast aspartyl protease yapsin 1.

Supplier: Bachem Americas

This fluorogenic HIV-1 protease substrate consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), attached at the COOH- and NH₂-termini. The γ-Abu spacer was inserted to avoid potential steric hindrance of substrate binding by the bulky acceptor. This FRET substrate is cleaved by the HIV-1 protease at the Tyr-Pro bond which results in a time-dependent increase in fluorescence intensity; excitation at 340 nm, emission at 490 nm.

Supplier: Bachem Americas

See also M-2460 and M-2465.

Supplier: Bachem Americas

The FA-peptides M-1400 and M-1410 are common ACE substrates and thus filed in the corresponding product family. For our 3-(2-furyl)acryloyl-amino acids (FA-amino acids) and the labeling reagent FA-OSu (Q-1245), please see the Amino Acids section.

Supplier: Bachem Americas

The FRET substrate Mca-RPKPYA-Nva-WMK(Dnp)-amide was hydrolyzed 60 times more rapidly by stromelysin 1 (MMP-3) (kcat/Km= 59400 M⁻¹s⁻¹) than by interstitial collagenase (MMP-1). However, it showed little discrimination between MMP-3, gelatinase A (MMP-2) (kcat/Km= 54000 M⁻¹s⁻¹), and gelatinase B (MMP-9) (kcat/Km= 55300 M⁻¹s⁻¹). Metalloelastase (MMP-12) digested this fluorogenic substrate with a kcat/Km of 243000 M⁻¹s⁻¹. For a fluorescent standard see M-2250.

Supplier: Bachem Americas

Sequence: H-Ala-βNA

Supplier: Bachem Americas

Bz-RGFFL-MNA, substrate for cathepsin D.

Supplier: Bachem Americas

Bachem additionally offers deuterated phenylalanine: Fmoc-[ring-D₅]Phe-OH B-4115.

Supplier: Bachem Americas

Sequence: H-Glu-pNA

Supplier: Bachem Americas

Suc-ALPF-pNA, substrate for FK-506 binding proteins (FKBPs, also called macrophilins) and cyclophilins, which belong to the group of peptidyl prolyl cis-trans isomerases (PPIases). This tetrapeptide has been used for an uncoupled protease-free assay of PPIase activity.

Supplier: Bachem Americas

Sequence: H-Leu-βNA

Supplier: Bachem Americas

Sequence: H-Ile-βNA

Supplier: Bachem Americas

Sequence: H-Trp-βNA

Supplier: Bachem Americas

Sequence: Bz-D-Arg-pNA · HCl

Supplier: Bachem Americas

Sequence: Z-Lys-OMe · HCl

Supplier: Bachem Americas

Sequence: Z-Lys-ONp · HCl

Supplier: Bachem Americas

Sequence: H-Leu-Gly-Gly-OH

Supplier: Bachem Americas

CALP-3, VKFGVGFK, acts as a calmodulin agonist. The octapeptide interacts with the calmodulin EF-hand motif, the Ca²⁺-binding site. CALP-3 activates phosphodiesterase in the absence of calcium and inhibits Ca²⁺-mediated cytotoxicity and apoptosis. CALP-3 is more active than CALP-1 (H-8155), By preventing trypsin activation within pancreatiic acinar cells CALP-3 shows potential therapeutic potential in the treatment of pancreatitis.

Supplier: Bachem Americas

This fluorescent (FRET) peptide substrate contains the wild-type amyloid precursor protein (APP) β-secretase cleavage site. Mca-SEVKMDAEFRK(Dnp)RR- amide has been used for assaying β-secretase-like activity of thimet oligopeptidase (TOP, EC 3.4.24.15). The results suggested that TOP is a potential β-secretase candidate and is involved in the processing of APP in vivo. See also L-1905.

Supplier: Bachem Americas

Intramolecularly quenched fluorescent substrate containing the ortho-aminobenzoyl (Abz) / N-(2,4-dinitrophenyl)ethylenediamine (EDDnp) groups as the donor / acceptor pair. It corresponds to the Swedish-mutated (JMV2236) β-amyloid precursor protein (βAPP) sequence targeted by β-secretase BACE (β-site APP-cleaving activity). This FRET substrate is more selectively cleaved by BACE1 and BACE2 than by cathepsin D, a disintegrin and metalloprotease 10 (ADAM10), tumor necrosis α-converting enzyme (TACE), presenilin-1 (PS1), or presenilin-2 (PS2).

Supplier: Bachem Americas

Sequence: H-Gly-Gln-OH

Supplier: Bachem Americas

AC-VEID-CHO, a specific inhibitor of caspase-6 (Mch2).

Supplier: Bachem Americas

Sequence: H-Leu-chloromethylketone · HCl

Supplier: Bachem Americas

Sequence: Z-Asp-2,6-dichlorobenzoyloxymethylketone
Synonym(s): Z-Asp-CH₂-DCB

Supplier: Bachem Americas

Sequence: ((RS)-2-Benzyl-3-mercaptopropionyl)-Gly-OH

Supplier: Bachem Americas

Sequence: 1-Methyl-1H-imidazole-2-carboxylic acid

Supplier: Bachem Americas

Sequence: 3-Palmitoyl-sn-glycerol
Synonym(s): L-α-Palmitin

Supplier: Bachem Americas

Sequence: 1,3-Dipalmitoyl-glycerol
Synonym(s): α,γ-Dipalmitin

Supplier: Bachem Americas

Sequence: 1,2-Distearoyl-sn-glycero-3-phosphocholine
Synonym(s): DSPC#L-β,γ-Distearoyl-α-lecithin