1910 Results for: "Anaspec"

Supplier: Anaspec

Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
NFF-3 is hydrolyzed rapidly by MMP-3 (kcat/Km = 218,000 s-1 M-1) and very slowly by MMP-9 (kcat/Km = 10,100 s-1 M-1), but no significant hydrolysis by MMP-1 and MMP-2. It is one of the few synthetic substrates selectively hydrolyzed by certain members of the MMP family and thus has important applications for the differentiation of MMP-3 activity from that of other MMPs. Abs/Em = 325/393 nm.
Sequence:Mca-RPKPVE-Nva-WR-K(Dnp)-NH2
MW:1675.9 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is an exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate: this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate.
Sequence:Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5
MW:655.9 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

In Alheimer's Disease brains, plaques contain amino-terminal truncated beta-Amyloid peptides including the alpha secretase-generated p3 fragments, beta-Amyloid 17-40 and 17-42. They were shown to induce pro-inflammatory cytokine and chemokine production in vitro and in vivo.

Supplier: Anaspec

This peptide corresponds to the CtoN inverted sequence of Beta-amyloid 1-42.
Sequence: AIVVGGVMLGIIAGKNSGVDEAFFVLKQHHVEYGSDHRFEAD
Molecular Weight: 4514.1 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: Anaspec

This kit is optimized to detect anti-human MOG (1-125) IgG in human samples. Wells are pre-coated with recombinant human MOG (1-125) protein and pre-blocked with proprietary solution. The amount of anti- human MOG IgG in serum or plasma is quantified using ELISA. Human anti-Human MOG (1-125) standard is included. Ample materials and reagents are provided to perform 96 assays.

Supplier: Anaspec

A glutathione-derived heavy metal-detoxifying peptide of higher plants consisting of 3 units of gammaGlu-Cys.
Sequence:(γE-C)3-G
MW:772.9 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

Rat adrenomedullin, rADM, (1-50) and its C-terminal rADM (11-50) induce a dose-dependent and endothelium-independent vasodilation on the arterial mesenteric vasculature.
Sequence:YRQSMNQGSRSTGCRFGTCTMQKLAHQIYQFTDKDKDGMAPRNKISPQGY-NH2 (Disulfide bridge: 14-19)
MW:5729.5 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C

Supplier: Anaspec

This peptide is a major constituent of protein deposits identified in the Islets of Langerhans of patients with noninsulin-dependent diabetes mellitus.
Sequence: KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY - NH2 (Disulfide bridge: 2 - 7)
MW: 3903.3 Da
% Peak Area by HPLC: ≥ 95%
Storage condition: -20°C

Supplier: Anaspec

The native peptide KKALRRQETVDAL (60249-1) is a selective substrate for Ca2+/calmodulin-dependent protein kinase (CaMK). The fluorescent and biotinylated peptides are used to design assays for CaMKs.
Sequence:KKALRRQETVDAL
MW:1527.8 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

A hypotensive and diuretic peptide. Originally isolated from the skin of the frog, Phyllomedusa sauvagei. It affects diuresis in the cardiovascular system and causes the release of ACTH and endorphins.
Sequence:Pyr-GPPISIDLSLELLRKMIEIEKQEKEKQQAANNRLLLDTI-NH2
MW:4599.4 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C

Supplier: Anaspec

Beta-Amyloid (1-42) peptide, a major component of amyloid plaques, accumulates in neurons of Alzheimer’s disease brains. Biochemical analysis of the amyloid peptides isolated from Alzheimer’s disease brain indicates that Beta-Amyloid (1-42) is the principal species associated with senile plaque amyloids, while Beta-Amyloid (1-40) is more abundant in cerebrovascular amyloid deposit.
Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Molecular Weight: 4514.1 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: Anaspec

Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is a sensitive substrate for assaying MMP-1, 2, 7, 8, 12 and 13 activities, Abs/Em = 494/521nm.
Sequence:5-FAM-RPKPYA-Nva-WM-K(QXL™ 520)-NH2
MW:2125.4 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

Streptavidin, nonglycosylated, tetrameric protein, 55,000-dalton, with each subunit able to bind a single molecule of the vitamin biotin, purified from Streptomyces avidinii, Physical State: Lyophilized powder, Solvent System: water, Storage -20 deg C desiccated, Size: 5mg

Supplier: Anaspec

Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
With the higher emission wavelength, this substrate is ideal for use in experiments where the test compounds might have strong autofluorescence at shorter wavelength, Abs/Em = 547/574 nm.
Sequence:QXL™ 570-KPLA-Nva-Dap(5-TAMRA)-AR-NH2
MW:1768 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is a sensitive substrate for assaying MMP-3 and 12 activities, Abs/Em = 494/521nm.
Sequence:5-FAM-RPKPVE-Nva-WRK(QXL™ 520)-NH2
MW:2143.3 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

Proinsulin C-peptide is the sequence of C peptide as it exists within proinsulin, the precursor to insulin which consists of insulin A chain, insulin B chain and C peptide (connecting peptide). In proinsulin, C peptide provides a means to ensure correct folding and assembly of the A and B chains. It is eventually cleaved away by proteases PC2, PC1/3 and CPE, at the flanking Arg-Arg and Lys-Arg basic residues (Arg-Arg-C peptide-Lys-Arg). Although C peptide is not present in mature insulin, it is stored in secretory granules, and eventually released into the bloodstream together with insulin in nearly equimolar amounts. Whereas insulin is metabolized quickly from circulation, C-peptide exhibits a slow turnover rate (>30 minutes). The measurement of the C-peptide is an important test for the β-cell function. In the red blood cells of type 2 diabetic patients, Na+,K+, ATPase activity is strongly related to blood C-peptide levels. C-peptide signal transduction in human renal tubular cells involves the activation of phospholipase C and PKC-δ and PKC-varepsilon, as well as RhoA, followed by phosphorylation of ERK1/2 and JNK and a parallel activation of Akt. C-peptide shows specific binding to a G-protein-coupled membrane binding site, resulting in Ca2+ influx, activation of mitogen-activated protein kinase signalling pathways and stimulation of Na+, K+ ATPase and endothelial nitric oxide synthase.
Sequence: RREAENPQAGAVELGGGLGGLQALALEGPPQKR
MW: 3340.7 Da
% Peak area by HPLC: 95
Storage condition: -20° C

Supplier: Anaspec

A control peptide for LSKL (inhibitor of thrombospondin).
Sequence:SLLK - NH2
MW:458.6 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C

Supplier: Anaspec

Despite the availability of alternative amine-reactive fluorescein derivatives that yield conjugates with superior stability and comparable spectra, fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. It appears that 5-FITC is more widely used than the 6-FITC isomer. FITC reagents are prominently used to label proteins. In addition, FITC has also been used to label peptides, oligonucleotides and other small organic ligands. A collection of recent applications is listed in the following references.

Supplier: Anaspec

This sequence corresponds to amino acids 3-13 from murine MBP. MBP induces immune reactivity in multiple sclerosis (MS), a chronic inflammatory demyelinating disease of the CNS. MBP (3-13) is a substrate for Protein Kinase C.
Sequence:QKRPSQRSKYL
MW:1390.6 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

PET (Positron Emission Tomography) imaging of [Lys3]-bombesin is able to detect gastrin-releasing peptide receptor (GRPR) positive prostate cancer. An immunoconjugate of [Lys3]-bombesin and corresponding monoclonal antibody can specifically induce (CD64)-dependent monocyte and neutrophil-mediated lysis of small cell carcinoma.
Sequence:Pyr-QKLGNQWAVGHLM-NH2
MW:1591.9 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

CREBtide is a synthetic substrate for PKA (Km = 3.9 µM). This peptide is based on the phosphorylation sequence in δ-CREB (cAMP response element binding protein).
Sequence:KRREILSRRPS-pY-RK
MW:1925.2 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

A number of Aß fragments including Aß (10-20) enhances aggregation of Aß (1-40). All the Aß peptides that enhance aggregation contain either residues 17 to 20 or 30 to 35, indicating the importance of these regions for promoting aggregation of full-length Aß.
Sequence: YEVHHQKLVFF
Molecular Weight: 1446.7 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: Anaspec

Matrix metalloproteinases (MMP’s) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-1 (collagenase-1) is involved in tumor development and metastasis and rheumatoid arthritis. It is proposed as a therapeutic target for these diseases. MMP-1 digests a broad range of substrates, including α-1 antitrypsin, myelin basic protein, collagen I, II, III, VII, VIII, casein, gelatin, and others

Supplier: Anaspec

This tridecapeptide, an alpha-factor pheromone of Saccharomyces cerevisiae, induces conjugation in yeast by binding to Ste2p. Its cognate GPCR activates a G protein signal pathway that highly conserves with mammalian signaling pathways.
Sequence:WHWLQLKPGQPMY
MW:1684 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec

Beta-amyloid N-terminally truncated (9-42) peptide. The degree of amino-terminal truncation varies in different neuritic plaque extractions; however, in studies of several Down’s syndrome patients subjected to neuritic plaque extraction, Aßs 1-42, 1-40, 3-42, 4-42, 5-42, 8-42, and 9-42 have been identified.
Sequence: GYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Molecular Weight: 3556.2 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: Anaspec

This serine substituted PLP (139-151) causes severe, acute experimental allergic encephalomyelitis in SJL mice.
Sequence: HSLGKWLGHPDKF
MW: 1521.8 Da
% Peak area by HPLC: 95
Storage condition: -20°C

Supplier: Anaspec

HLA-A11 restricted epitope from Epstein-Barr Virus BRLF1 (134-142).
Sequence: ATIGTAMYK
MW: 955.2 Da
% Peak area by HPLC: 95
Storage condition: -20°C

Supplier: Anaspec

Rat ANP differs from the human hormone by only one residue at position 12. ANP (1-28) peptide hormone constitutes the first 28 amino acids of the ANP synthesized in the heart of different vertebrates. It plays an important role in the regulation of blood pressure and natriuresis/diuresis. Residues Phe8, Arg14 and the C-terminal sequence of ANP are known to bind to human NPR-A (Natriuretic peptide receptor type A).
Sequence:SLRRSSCFGGRIDRIGAQSGLGCNSFRY (Disulfide bridge: 7-23)
MW:3062.5 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C

Supplier: Anaspec

One of the three mammalian Natriuretic peptides, A-type is the Atrial natriuretic peptide (ANP) also called Cardiodilatin (CDD). ANP (1-28) peptide hormone constitutes the first 28 amino acids of the ANP synthesized in the heart of different vertebrates. It plays an important role in the regulation of blood pressure and natriuresis/diuresis. Residues Phe8, Arg14 and the C-terminal sequence of ANP are known to bind to human NPR-A (Natriuretic peptide receptor type A).
Sequence:SLRRSSCFGGRMDRIGAQSGLGCNSFRY (Disulfide bridge: 7-23)
MW:3080.5 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C

Supplier: Anaspec

A potent peptide inhibitor of Hepatitis Virus C NS3 protease.