1911 Results for: "Anaspec"

Supplier: Anaspec Inc

Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester, Biotin, NHS ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.

Supplier: Anaspec Inc

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I

Supplier: Anaspec Inc

Enterokinase is a heterodimeric serine protease produced by cells in the duodenal wall

Supplier: Anaspec Inc

Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho-Serine/Threonine-Proline peptide bonds

Supplier: Anaspec Inc

ADAM10 has been shown to be an effective α-secretase in vitro and in vivo

Supplier: Anaspec Inc

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components

Supplier: Anaspec Inc

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components

Supplier: Anaspec Inc

Cathepsin G is the serine protease released by neutrophils upon their activation

Supplier: Anaspec Inc

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components

Supplier: Anaspec Inc

Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is a sensitive substrate for assaying MMP-1, 2, 7, 8, 12 and 13 activities, Abs/Em = 494/521 nm.
Sequence:5-FAM-PLGL-Dap(QXL™ 520)-AR-NH2
MW:1560.6 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec Inc

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria

Supplier: Anaspec Inc

Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7

Supplier: Anaspec Inc

The SensoLyte® 520 Acetylcholinesterase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of AChE inhibitors

Supplier: Anaspec Inc

DPPIV is a widely distributed serine protease that cleaves two amino acids from small peptides containing alanine or proline in the second position of the N-terminus of the peptide

Supplier: Anaspec Inc

Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily, playing an important role in host defense against cancer cells in addition to it’s roles in protein degradation, antigen processing etc

Supplier: Anaspec Inc

Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases

Supplier: Anaspec Inc

Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption, also having a potential as a drug target in autoimmune diseases and osteoporosis

Supplier: Anaspec Inc

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.

Supplier: Anaspec Inc

TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

Supplier: Anaspec Inc

The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 /520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.

Supplier: Anaspec Inc

MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease

Supplier: Anaspec Inc

Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators.

Supplier: Anaspec Inc

The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format.

Supplier: Anaspec Inc

Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity

Supplier: Anaspec Inc

GST (Glutathione S-Transferase) enzymes, a family of isozymes plays a crucial role in body defense mechanisms against toxins and carcinogens

Supplier: Anaspec Inc

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins

Supplier: Anaspec Inc

Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown

Supplier: Anaspec Inc

β-galactosidase, encoded by the lacZ gene in E

Supplier: Anaspec Inc

Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme

Supplier: Anaspec Inc

MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens