"Advanced Biomatrix"
Poly-L-Ornithine
Supplier: Advanced Biomatrix
Poly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine. Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.
The molecular weight of Poly-L-Ornithine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
Poly-L-Ornithine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.
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PureCol® Type I Collagen Coated Well Plates, Advanced BioMatrix
Supplier: Advanced Biomatrix
Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.
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PureCol® Bovine Collagen Type I
Supplier: Advanced Biomatrix
PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
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Phosphate Buffered Saline, 10X, Advanced Biomatrix
Supplier: Advanced Biomatrix
Use With Type I Collagen to help form 3D hydrogels.
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Hydrochloric acid 0.01M, sterile-filtered
Supplier: Advanced Biomatrix
HCl is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
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Sodium hydroxide 0.1M, sterile-filtered
Supplier: Advanced Biomatrix
NaOH is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
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ECM Select® Array Kit Ultra- Extracellular Matrix Screening Array, 36 Conditions, Advanced BioMatrix
Supplier: Advanced Biomatrix
Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in your research.
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Mebiol® Thermoreversible Hydrogel, Advanced BioMatrix
Supplier: Advanced Biomatrix
Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.
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CytoSoft® Discovery Kit, Tunable Substrate Stiffness,Of Each Stiffness, Advanced BioMatrix
Supplier: Advanced Biomatrix
Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.
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Bovine Collagen Type I (Methacrylated)
Supplier: Advanced Biomatrix
Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.
The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the e-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Approximately 40% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml.The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.
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Hyaluronic acid sodium salt methacrylated, PhotoHA®
Supplier: Advanced Biomatrix
Methacrylated Hyaluronic Acid (HA Only)
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Native TeloCol®-10 Bovine Native Collagen, Type I (from Hide)
Supplier: Advanced Biomatrix
TeloCol®-10 is 20 mL of 10 mg/ml, type I bovine telocollagen solution for 2D coatings or 3D hydrogels. TeloCol®-10 has a high viscosity and concentration, and can form firm hydrogels.
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Chloro(pentamethylcyclopentadienyl)bis(triphenylphosphine)ruthenium(II) photoinitiator, for visible light crosslinking
Supplier: Advanced Biomatrix
Ruthenium Visible Light Photoinitiator (400-450nm)
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CytoSoft® Imaging 96-well Plates, Advanced BioMatrix
Supplier: Advanced Biomatrix
CytoSoft® Imaging products provide a tool to culture cells on PDMS substrates with various rigidity including 0.2, 0.5, 2, 8, 16, 32, and 64 kPa. This CytoSoft® Imaging plate comes with 1 glass-bottom 96-well plate for high resolution imaging of cells on the selected stiffness.
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5-FAM Conjugate Collagen Hybridizing Peptide (Green), Advanced Biomatrix
Supplier: Advanced Biomatrix
CHP can specifically bind to denatured collagen.
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Biotin Conjugate Collagen Hybridizing Peptide (Biotin), Advanced Biomatrix
Supplier: Advanced Biomatrix
CHP can specifically bind to denatured collagen.
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