"Advanced Biomatrix"

Supplier: Advanced Biomatrix

Ruthenium Visible Light Photoinitiator (400-450nm)

Supplier: Advanced Biomatrix

Tropoelastin is the precursor to the elastin molecule. Elastin is found in many tissues of the body including the skin, arterial walls and ligaments. Elastin confers strength and elasticity to tissues and organs.

Tropoelastin is not normally available in its native state since it becomes cross-linked immediately after its synthesis by the cell, during its export into the extracellular matrix.

This tropoelastin is produced using recombinant production methods. The product is supplied as a sterile, lyophilized powder containing 1 mg per vial. A Certificate of Analysis is available with the purchase of each product.

Tropoelastin is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation.

Supplier: Advanced Biomatrix

E-Cadherin has been used as coating matrix protein for 1) maintaining long-term ES or iPS cell culture then combine with proper ES cell culture media, 2) as a coating matrix material for 11R tag recombinant TF intracellular delivery for protein derived iPS protocol with extremely low-level non-specific interaction and 3) as a native antigen for antibody production.

Supplier: Advanced Biomatrix

CHP can specifically bind to denatured collagen.

Supplier: Advanced Biomatrix

TeloCol®-6 bovine collagen solution is derived from an acid extraction process yielding a telopeptide-intact collagen. The pro-peptide regions at both ends of the collagen chain, N- and C-telopeptide regions, are maintained.

TeloCol®-6 collagen is approximately 95% Type I collagen with the remainder being comprised of Type III collagen. Each product includes a bottle containing 50 ml of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This product is supplied at a concentration of approximately 6 mg/ml with the concentration for each specific lot provided on the product label and Certificate of Analysis that is available with the purchase of each product. TeloCol®-6 is sterile filtered and provided in user-friendly packaging for use and storage.

TeloCol®-6 is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

Supplier: Advanced Biomatrix

PhotoCol®-IRG is a methacrylated Type I collagen kit. These 3D collagen gels can be prepared at various concentrations and photocrosslinked (365nm) to provide various gel stiffness. Each kit contains 100 mg of methacrylated collagen, irgacure photoinitiator, acetic acid and a neutralization solution.

Supplier: Advanced Biomatrix

PhotoGel® is 1 gram (2 x 500 mg) of lyophilized methacrylated gelatin. PhotoGel® provides 3D gelatin hydrogels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

Supplier: Advanced Biomatrix

Advanced BioMatrix products are recognized for purity, functionality, quality and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: Advanced Biomatrix

Collagenase consists of a blend of enzymes including collagenase, caseinase, clostripain and trypsin designed to effectively hydrolyze collagen and dissociate tissues. Various collagenase products have been evaluated specifically for use with Advanced BioMatrix’ s collagen products. This product is offered to improve the viability and functionality of isolated cells from the digested collagen gels.

Collagenase is provided as a lyophilized, sterile powder in a 50 mg package size. After reconstitution, the product is ready-to-use.

Supplier: Advanced Biomatrix

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: Advanced Biomatrix

Poly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine. Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.

The molecular weight of Poly-L-Ornithine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Ornithine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

Supplier: Advanced Biomatrix

VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. VitroCol®, lyophilized form is not recommend for the formation of a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is supplied as a sterile, lyophilized powder.

Supplier: Advanced Biomatrix

LAP is a water soluble photoinitiator for use in the polymerization of hydrogels or bioinks. LAP is photocrosslinked with 405nm blue light and is often preferred over Irgacure 2959 due to improved cytocompatibility.

Supplier: Advanced Biomatrix

Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds.

Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs).

Fibronectin is ideal for coating of surfaces and is typically used at a concentration of 1-5 µg/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a sterile, lyophilized product.

Supplier: Advanced Biomatrix

RatCol® Type I Acid Soluble Rat Tail Collagen contains 100 mg at a concentration of approximately 4 mg/mL in a 0.02M acetic acid solution (pH 2 to 3). RatCol® collagen is soluble telo-collagen. Each product includes a bottle containing 100 mg of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This collagen product is provided in user-friendly packaging for use and storage. This product is sterile filtered and is supplied as a ready to use solution. The concentration for each specific lot is provided on the product label and on a Certificate of Analysis that is available with the purchase of each product.

This product is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. RatCol® collagen is suitable for applications using a variety of cell lines including hepatocytes, fibroblasts and epithelial cells.

Supplier: Advanced Biomatrix

Type I bovine collagen, lyophilized fibrous powder is extracted from bovine flexor tendon with the raw material sourced from closed/controlled herds of animals. Because the collagen is extracted from tendon, the material is more naturally crosslinked. The collagen is polymeric insoluble collagen. The manufacturing processes comply with stringent quality standards that have proven to yield a high quality product with lot-to-lot consistency. This product has a purity of >96% with Type II and Type III collagens not detectable.

This product is supplied as a lyophilized fibrous powder in a 1 gram package size. Bioburden and endotoxin levels are tested – this product is not considered sterile.

This collagen product is naturally cross-linked yielding a robust material for applications which require structure and strength. This product can be readily prepared into such forms as tissue scaffolds, foams, sponges, suspensions, coatings, putties, films and sheets but does not form hydrogels. Using typical cross-linking methods, this material can be tuned for optimal in vivo resorption. This collagen product is ideal for tissue engineering applications and uses with inorganic and biomaterials.

The product is provided in user-friendly packaging for use and storage. Avoid extended exposure to ambient environment since this material is hygroscopic.