"Advanced Biomatrix"

Supplier: Advanced Biomatrix

HyStem®-C Hydrogel Kits - The starter matrix.

Supplier: Advanced Biomatrix

Type IV Collagen is the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha 1-like and one Alpha 2-like chains.

This Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a sterile, lyophilized powder containing 5 mg of Type IV collagen per vial. A Certificate of Analysis is available with the purchase of each product.

Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.

Supplier: Advanced Biomatrix

CytoSoft® Imaging products provide a tool to culture cells on PDMS substrates with various rigidity including 0.2, 0.5, 2, 8, 16, 32, and 64 kPa. This CytoSoft® Imaging plate comes with 1 glass-bottom 96-well plate for high resolution imaging of cells on the selected stiffness.

Supplier: Advanced Biomatrix

NaOH is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

Supplier: Advanced Biomatrix

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: Advanced Biomatrix

PhotoGel®-LAP is a methacrylated gelatin kit. These 3D gelatin hydrogels can be prepared at various concentrations and photocrosslinked (405nm) to provide various gel stiffness. Each kit contains 1 gram (2 x 500 mg) of methacrylated gelatin and LAP photoinitiator.

Supplier: Advanced Biomatrix

Use With Type I Collagen to help form 3D hydrogels.

Supplier: Advanced Biomatrix

Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.

The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the e-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Approximately 40% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml.The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

Supplier: Advanced Biomatrix

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in your research.

Supplier: Advanced Biomatrix

Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.
The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. Poly-D-Lysine is supplied in a sterile 5 mg package size.

Supplier: Advanced Biomatrix

Type IV Collagen is the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha 1-like and one Alpha 2-like chains.

This Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a non-sterile powder containing 5 mg of Type IV collagen per vial. A Certificate of Analysis is available with the purchase of each product.

Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.

Supplier: Advanced Biomatrix

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: Advanced Biomatrix

Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.

Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.

Supplier: Advanced Biomatrix

PhotoCol®-RUT is a methacrylated Type I collagen kit. These 3D collagen gels can be prepared at various concentrations and photocrosslinked (400-450nm) to provide various gel stiffness. Each kit contains 100mg of methacrylated collagen, ruthenium photoinitiator, acetic acid and a neutralization solution.

Supplier: Advanced Biomatrix

Advanced BioMatrix products are recognized for purity, functionality, quality and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: Advanced Biomatrix

PhotoCol®-LAP is a methacrylated Type I collagen kit.These 3D collagen gels can be prepared at various concentrations and photocrosslinked (405nm) to provide various gel stiffness. Each kit contains 100 mg of methacrylated collagen, LAP photoinitiator, acetic acid and a neutralization solution.