Acetonitrile ≥99.9% for LC-MS, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce Acetonitrile (ACN) is an LC/MS-grade preparation with high purity and low UV-absorptivity that makes it suitable for HPLC and mass spectrometry applications.
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Protector® Demonstration Hoods, Labconco®
Supplier: Labconco
Designed for classroom use, these lightweight hoods feature an epoxy-coated aluminum frame with tempered safety glass front sash, sides, rear baffle, and top.
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FilterMate™ Portable Exhausters, Labconco®
Supplier: Labconco
Portable exhausters for use with XPert™ balance enclosures and weigh boxes, Precise™ HEPA-filtered glove boxes, and Protector™ XVS ventilation stations, demonstration hoods, and workstations. Exhausters use carbon and/or HEPA filters to remove hazardous powders, particulates, or vapors from the exhaust air stream, returning filtered air to the environment. No ducting to the outside is required.
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Dimethyl sulfoxide for molecular biology
Supplier: MP Biomedicals
Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.