Supplier: ADVANCED BIOMATRIX, INC. MS

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: ADVANCED BIOMATRIX, INC. MS

Advanced BioMatrix products are recognized for purity, functionality, quality and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: ADVANCED BIOMATRIX, INC. MS

Poly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine. Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.

The molecular weight of Poly-L-Ornithine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Ornithine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

Supplier: ADVANCED BIOMATRIX, INC. MS

PhotoGel® is 1 gram (2 x 500 mg) of lyophilized methacrylated gelatin. PhotoGel® provides 3D gelatin hydrogels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

Supplier: ADVANCED BIOMATRIX, INC. MS

LAP is a water soluble photoinitiator for use in the polymerization of hydrogels or bioinks. LAP is photocrosslinked with 405nm blue light and is often preferred over Irgacure 2959 due to improved cytocompatibility.

Supplier: ADVANCED BIOMATRIX, INC. MS

Collagenase consists of a blend of enzymes including collagenase, caseinase, clostripain and trypsin designed to effectively hydrolyze collagen and dissociate tissues. Various collagenase products have been evaluated specifically for use with Advanced BioMatrix’ s collagen products. This product is offered to improve the viability and functionality of isolated cells from the digested collagen gels.

Collagenase is provided as a lyophilized, sterile powder in a 50 mg package size. After reconstitution, the product is ready-to-use.

Supplier: ADVANCED BIOMATRIX, INC. MS

VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. VitroCol®, lyophilized form is not recommend for the formation of a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is supplied as a sterile, lyophilized powder.

Supplier: ADVANCED BIOMATRIX, INC. MS

Sterile filtered, degassed, deionized water at pH 5.0-8.0. This product was designed and intended for use with the HyStem® family of thiolated hyaluronic acid products. 

Supplier: ADVANCED BIOMATRIX, INC. MS

PhotoCol®-RUT is a methacrylated Type I collagen kit. These 3D collagen gels can be prepared at various concentrations and photocrosslinked (400-450nm) to provide various gel stiffness. Each kit contains 100mg of methacrylated collagen, ruthenium photoinitiator, acetic acid and a neutralization solution.

Supplier: ADVANCED BIOMATRIX, INC. MS

CytoSoft® Imaging products provide a tool to culture cells on PDMS substrates with various rigidity including 0.2, 0.5, 2, 8, 16, 32, and 64 kPa. This CytoSoft® Imaging plate comes with 1 glass-bottom 96-well plate for high resolution imaging of cells on the selected stiffness.

Supplier: ADVANCED BIOMATRIX, INC. MS

Type IV Collagen is the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha 1-like and one Alpha 2-like chains.

This Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a sterile, lyophilized powder containing 5 mg of Type IV collagen per vial. A Certificate of Analysis is available with the purchase of each product.

Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.

Supplier: ADVANCED BIOMATRIX, INC. MS

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: ADVANCED BIOMATRIX, INC. MS

Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.

Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.

Supplier: ADVANCED BIOMATRIX, INC. MS

NaOH is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

Supplier: ADVANCED BIOMATRIX, INC. MS

HyStem®-C Hydrogel Kits - The starter matrix.

Supplier: ADVANCED BIOMATRIX, INC. MS

PhotoGel®-LAP is a methacrylated gelatin kit. These 3D gelatin hydrogels can be prepared at various concentrations and photocrosslinked (405nm) to provide various gel stiffness. Each kit contains 1 gram (2 x 500 mg) of methacrylated gelatin and LAP photoinitiator.

Supplier: ADVANCED BIOMATRIX, INC. MS

Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.

The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the e-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Approximately 40% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml.The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

Supplier: ADVANCED BIOMATRIX, INC. MS

Use With Type I Collagen to help form 3D hydrogels.

Supplier: ADVANCED BIOMATRIX, INC. MS

Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.
The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. Poly-D-Lysine is supplied in a sterile 5 mg package size.

Supplier: ADVANCED BIOMATRIX, INC. MS

Type IV Collagen is the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha 1-like and one Alpha 2-like chains.

This Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a non-sterile powder containing 5 mg of Type IV collagen per vial. A Certificate of Analysis is available with the purchase of each product.

Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.

Supplier: ADVANCED BIOMATRIX, INC. MS

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in your research.

Supplier: ADVANCED BIOMATRIX, INC. MS

Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Supplier: ADVANCED BIOMATRIX, INC. MS

PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.

PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

Supplier: ADVANCED BIOMATRIX, INC. MS

PureCol® -S collagen is provided as a standard for use in assays where ultra-pure collagen is required. PureCol®-S collagen standard is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. This product is supplied at approximately a 3 mg/ml concentration. The concentration is confirmed by Biuret protein determination assay. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol®-S is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

PureCol® -S is ideal for using as a collagen standard in controlled testing and assay procedures. PureCol®-S is sterile filtered and is supplied as a ready to use solution.

Supplier: ADVANCED BIOMATRIX, INC. MS

PhotoHA®-IRG is a methacrylated hyaluronic acid (HAMA) kit. These 3D hyaluronic acid hydrogels can be prepared at various concentrations and photocrosslinked (365nm) to provide various gel stiffness. Each kit contains 100 mg of methacrylated hyaluronic acid and irgacure photoinitiator.

Supplier: ADVANCED BIOMATRIX, INC. MS

Type III Collagen provides structure and strength to connective tissue, is found many places in the body, especially skin, lung, intestinal walls and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. The Type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen’s tissue strengthening function.

This Type III Collagen product is isolated from human placenta and is purified using a multi-step process with approximately 85% Type III collagen with the remainder being comprised of Type I collagen. The product is supplied as a non-sterile powder. A Certificate of Analysis is available with the purchase of each product. Type III collagen product is provided in a user-friendly packaging for use and storage.

Type III Collagen is typically used as a thin coating on tissue culture surfaces or as a control. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type III collagen may be used to culture a variety of cell types.

Supplier: ADVANCED BIOMATRIX, INC. MS

Methacrylated Hyaluronic Acid with Ruthenium Kit

Supplier: ADVANCED BIOMATRIX, INC. MS

Nutragen® collagen is known as the standard of all collagens for purity (>99% collagen content), functionality, and the most native-like collagen available. Nutragen® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

Nutragen® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.

Nutragen® collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. Nutragen® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

Nutragen® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Nutragen® collagen is provided in a 50 mL volume and is contained in user-friendly packaging for use and storage. Nutragen® is sterile filtered and is supplied as a ready to use solution.

Supplier: ADVANCED BIOMATRIX, INC. MS

Methacrylated Hyaluronic Acid with LAP Kit

Supplier: ADVANCED BIOMATRIX, INC. MS

PhotoGel®-RUT is a methacrylated gelatin kit. These 3D gelatin hydrogels can be prepared at various concentrations and photocrosslinked (400-450nm) to provide various gel stiffness. Each kit contains 1 gram (2 x 500 mg) of methacrylated gelatin and ruthenium photoinitiator.