19840 Results for: "4,4-Dimethyl-3-thiosemicarbazide"
P450-Glo CYP2C19 Screening System, Promega
Supplier: Promega Corporation
The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
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P450-Glo CYP2B6 Screening System, Promega
Supplier: Promega Corporation
The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
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P450-Glo CYP3A4 Assay (Luciferin-PPXE) DMSO-Tolerant Assay, Promega
Supplier: Promega Corporation
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
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P450-Glo CYP3A4 Screening System (Luciferin-PPXE) DMSO-Tolerant Assay, Promega
Supplier: Promega Corporation
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
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Ultra-Low Temperature Freezers, TSX Universal −86 °C ULT
Supplier: Thermo Fisher Scientific
TSX Universal series ULT freezers are the pinnacle of reliability, sustainability, and innovation incorporating insights from over 80 years of experience to enhance every aspect of the system. Updates to the variable speed compressors has made the TSX Universal adaptable across all workflows. Available in four capacities, they are ENERGY STAR® certified with a 33% energy efficiency improvement, ACT labeled and produced in a Zero-Waste to Landfill certified facility.
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Purifier® Vertical Clean Benches, Labconco®
Supplier: Labconco
Clean benches provide product protection from environmental contaminants for applications requiring a particulate-free work area, including plant tissue culture, electronic part inspection, syringe filling, medical device assembly, media plate preparation, and PCR
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Sulfo-SMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
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Sulfo-DIBMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
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Sodium deoxycholate monohydrate 98%
Supplier: Thermo Scientific Chemicals
98%. 100g.