DNase I

Degrade dsDNA and ssDNA without damaging important RNA in your sample or reaction

• Digest away unwanted DNA without digesting RNA
• Use in a variety of applications such as in vitro transcription and RT-PCR

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation

Applications include:
Elimination of template DNA following in vitro synthesis of RNA with T7, SP6, or T3 RNA polymerase
Labeling of DNA by nick translation, in combination with Klenow or other DNA polymerases
Treatment of RNA prior to RT-PCR
Characterization of DNA-protein interactions by DNase I footprinting

It efficiently hydrolyzes dsDNA and ssDNA into a mixture of short oligonucleotides and mononucleotides.

Certifications: For research use only. Not for human or diagnostic use.

Ordering information: Contents include enzyme and a 10X reaction buffer. Units reported are MBU or Molecular Biology Units. See the product user manual for the definition of MBU.