AffinityScript cDNA Synthesis Kits, Agilent Technologies
Product Details & Documents
Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications.
- Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 min with linear detection from 3 pg to 3 µg total RNA
- Creates up to 12 kb cDNA in 25 min or less
- Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The streamlined protocol produces cDNA in 15 min with linear detection from 3 pg to 3 µg of total RNA. The easy-to-use process generates up to 12 kb cDNA in 25 min or less. Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format, using SYBR Green or probe-based detection.
The AffinityScript Multiple Temperature cDNA synthesis kit contains Agilent’s popular, highly thermostable, qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase for robust yields, from 37 to 60 °C, with nearly full activity between 45 to 55 °C. With the AffinityScript multiple temperature cDNA synthesis kit, Agilent offers enzymes with a broad thermal profile.
The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.
The system’s multi-temperature capability allows you to change RT reaction temperature without having to change the reverse transcriptase, for a faster and more efficient analysis. In addition, when amplifying low RNA input amounts and resolving RNA secondary structures it delivers high cDNA yields, and can operate effectively at temperatures up to 55 °C.
Caution: For research use only. Not for use in diagnostic procedures.
Specifications
Specifications
- Description:AffinityScript multiple temperature reverse transcriptase
- No. of reactions:50
- Details:Engineered to be highly thermostable, allowing you to reverse transcribe at your preferred reaction temperature. Achieve a robust cDNA yield with even GC-rich sequences. Increased activity at all temperatures in a broad operating range, from 37 to 55 °C. The system is able to synthesize cDNA up to 20 kb
Specifications
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