55C MMLV-RT
About this item
A highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.
- Thermostable reverse transcriptase activity up to 60 °C
- Ideal for cDNA synthesis from RNA with high secondary structure such as viral RNA genomes
- Sensitive detection of low copy number RNA targets
- Perfect for developing fast, highly reproducible RT-qPCR assays
For cDNA synthesis, a higher reaction temperature is sometimes desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding. 55C MMLV-RT is a highly thermostable reverse transcriptase with reduced RNase H activity, that allows very efficient first-strand cDNA synthesis at temperatures up to 60 °C. This improves the cDNA yield from difficult RNA targets, such as viral RNA genomes, that require higher temperature to denature strong RNA secondary structure.
: ISO 13485
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
A highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.
- Thermostable reverse transcriptase activity up to 60 °C
- Ideal for cDNA synthesis from RNA with high secondary structure such as viral RNA genomes
- Sensitive detection of low copy number RNA targets
- Perfect for developing fast, highly reproducible RT-qPCR assays
For cDNA synthesis, a higher reaction temperature is sometimes desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding. 55C MMLV-RT is a highly thermostable reverse transcriptase with reduced RNase H activity, that allows very efficient first-strand cDNA synthesis at temperatures up to 60 °C. This improves the cDNA yield from difficult RNA targets, such as viral RNA genomes, that require higher temperature to denature strong RNA secondary structure.
: ISO 13485
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 78577-120
- 78577-106
- Format:
- Enzyme with/without buffer
- Enzyme with/without buffer
- Features:
- Glycerol free
- Glycerol free
- Hot start:
- No
- No
- High fidelity:
- No
- No
- Amplicon Size:
- ≤12 kb
- ≤12 kb
- Processivity:
- Fast
- Fast
- Product overhang:
- No
- No
- Storage Conditions:
- –20 °C
- –20 °C
- Application:
- Inhibitor rich sample
- Inhibitor rich sample
Specifications
Product Family Options
Product Information
- ConcentrationReaction volumeUnitsVolumeNo. of reactionsSizeAvailabilityPrice
- 78577-12055C MMLV-RTSupplier Number: MDX117-005Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:New Product
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Specifications
Cat. No.78577-120FormatEnzyme with/without bufferFeaturesGlycerol freeHot startNoHigh fidelityNoAmplicon Size≤12 kbProcessivityFastProduct overhangNoStorage Conditions–20 °CApplicationInhibitor rich sampleNew Product - 78577-10655C MMLV-RTSupplier Number: MDX117-02Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:New Product
More
Specifications
Cat. No.78577-106FormatEnzyme with/without bufferFeaturesGlycerol freeHot startNoHigh fidelityNoAmplicon Size≤12 kbProcessivityFastProduct overhangNoStorage Conditions–20 °CApplicationInhibitor rich sampleNew Product