CTGF (Full) ELISA Kit Wako

This kit quantifies CTGF (Full) in human serum/plasma. By using antibodies targeting Module 1 and Module 4 of CTGF, it allows measurement of total CTGF including cleaved forms.

• When combined with the CTGF (Full+N) ELISA Kit (292-84901), specific quantification of N-terminal CTGF is possible - useful for IPF (Idiopathic pulmonary fibrosis) and fibrotic disease research for human serum/plasma(EDTA)

CTGF is a secretory protein of approximately 38 kDa produced by umbilical vein and vascular endothelial cells. CTGF is composed of 4 domains, designated Modules 1 through 4. Module 1 is the IGF-binding domain, Module 2 the vWC domain, Module 3 the TSP-1 domain, and Module 4 the CT domain, and different factors bind to each domain. CTGF is reportedly involved in the proliferation and differentiation of chondrocytes and in cell adhesion, as well as being a major factor in tissue fibrosis, and has been proposed as a candidate biomarker for various fibrotic disorders. Meanwhile, it has been reported that CTGF in blood contains an N-terminal region in which the C-terminal region has been cleaved, giving rise to the problem that the amount of N-terminal CTGF in blood cannot be measured because it is mixed with platelet-derived full-length CTGF at the time of blood sample collection1).

N-terminal CTGF has been proposed as a potential biomarker for pulmonary fibrosis2). This product is an ELISA kit for detection of CTGF (Full) using monoclonal antibodies that recognize CTGF Module 1 and Module 4. The amount of N-terminal CTGF in blood can be measured when this product is used in combination with a kit for detecting CTGF (Full) and CTGF (N-terminal region) (Code No. 292-84901 CTGF (Full + N-terminal region) ELISA Kit Wako)1). 1) Miyazaki, O. et al. : Ann. Clin. Biochem., 47, 205 (2010). 2) Kono, M. et al. : Clin. Chim. Acta, 412, 2211 (2011).