FTO RNA demethylase is named the fat mass and obesity-associated protein (FTO) due to its association with human obesity.

• Catalyzes the demethylation of N6-methyladenosine (m6A) on RNA to form Adenosine

• An RNA-seq product for m6A mapping of RNA
• Demethylates N6-methyl-deoxyadenosine (6 mA) and 3-methylthymine (3 mT) on ssDNA

This enzyme is a homolog of the Fe(II)/ alpha-ketoglutarate – dependent AlkB dioxygenases and is an N6-methyladenosine (m6A) demethylase of eukaryotic mRNA. In vivo, FTO demethylates internal m6A and cap N6, 2 prime-O-dimethyladenosine (m6Am) in mRNA and snRNA. FTO also mediates N1-methyladenosine (m1A) demethylation in tRNA. In vitro, FTO demethylates m6A, m6Am and m3U in ssRNA, as well as N6-methyl-deoxyadenosine (6 mA) and 3-methylthymine (3 mT) on ssDNA.