About this item
IL-3 is a species-specific cytokine and pleiotropic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages. Produced by T cells, mast cells, and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
High quality
Low endotoxin
Lot-to-lot consistency
Animal free
Manufactured following GMP guidelines
Host cell protein: ≤30 ng/ml, host cell DNA: ≤0.01 ng/ml, sterility testing: no growth, mycoplasma: negative.
Specifications
- Conjugation:Unconjugated
- Protein Function:Cytokine
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Storage Conditions:At or below –20 °C
- Endotoxin Content:Endotoxin LAL (EU/µg) ≤0.05
- Biological Activity:Bioactivity assay: Proliferation of TF-1; ED50 ≤2 ng/ml; Bioactivity ≥4.2 x10³ IU/µg
- Gene ID:P08700
- Reconstitution Instructions:Sterile water at 0.1 - 0.5 mg/ml
- Endotoxin-free:N
- Carrier-Free:Y
- Protease-free:N
- Animal-Free:Y
- Protein Synonyms:MCGF|Multi-CSF|HCGF|P-cell stimulating factor
- Protein/Peptide Name:IL-3
- Purity:≥97%
- Molecular Weight:15.2 kDa
- Sequence:MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
- Endotoxin Level:Low
- Formulation:10 mM sodium phosphate, 150 mM sodium chloride, pH 7.5
- Nuclease-free:N
- Grade:GMP
- Shipping Temperature:Ambient
- Tested Applications:Bioassay