IL-4 is an immunomodulatory cytokine that stimulates B cell proliferation and activates eosinophils, basophils, and mast cells. Induces vascular cell adhesion molecule (VCAM)-1 expression on endothelial cells and involved in collagen production by fibroblasts. Plays a key role in promotion of Th2 differentiation from naïve helper T cells. Defines the Th2 phenotype of lymphocytes. Regulates cell proliferation, apoptosis, and expression of numerous genes in various cell types. The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
- High quality Low endotoxin Lot-to-lot consistency Animal free Manufactured following GMP guidelines
Host cell protein: ≤30 ng/ml, host cell DNA: ≤0.01 ng/ml, sterility testing: no growth, mycoplasma: negative.
- Conjugation:Unconjugated
- Protein Function:Cytokine
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Storage Conditions:At or below –20 °C
- Endotoxin Content:Endotoxin LAL (EU/µg) ≤0.05
- Biological Activity:Bioactivity assay:TF-1 cell proliferation; ED50 ≤0.25 ng/ml; Bioactivity ≥1.4×10⁴ IU/µg
- Gene ID:P05112
- Reconstitution Instructions:Sterile water at 0.1 - 0.5 mg/ml
- Endotoxin-free:N
- Carrier-Free:Y
- Protease-free:N
- Animal-Free:Y
- Protein Synonyms:B-cell stimulatory factor-1|BSF-1|BCDF|BCGF
- Protein/Peptide Name:IL-4
- Purity:≥98%
- Molecular Weight:15.1 kDa
- Sequence:MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
- Endotoxin Level:Low
- Formulation:20 mM sodium phosphate, pH 7.5
- Nuclease-free:N
- Grade:GMP
- Shipping Temperature:Ambient
- Tested Applications:Bioassay