BalanCD™ HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.

• Increase viable cell density and culture duration to maximize yield Use the same Feed for a wide range of HEK293 cell lines Fulfill regulatory requirements Chemically-defined, animal component-free formula Drug Master File (DMF) submitted to US FDA

The BalanCD HEK293 System is a chemically defined, animal component-free platform of media and supplement optimized for production of viral vectors and transient protein expression. The product system comprises of BalanCD HEK293 medium, BalanCD HEK293 Feed, and Anti-Clumping Supplement. BalanCD HEK293 System contains no hydrolysates, L-Glutamine, antibiotics, antimycotics, or any other undefined components, and is ready to use for suspension culture applications. BalanCD HEK293 medium contains 6 g/L glucose and BalanCD HEK293 Feed contains 40 g/L glucose. BalanCD HEK293 medium can be supplemented with BalanCD HEK293 Feed for high density cell culture, or with Anti-Clumping Supplement post-transfection to minimize cell aggregation.