Specifications
- Antibody Type:Primary
- Antigen Name:Intercellular Adhesion molecule 1
- Antigen Symbol:ICAM1
- Clonality:Monoclonal
- Clone:MEM-111
- Conjugation:Unconjugated
- ELISA:Yes
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoPrecipitation:Yes
- Isotype:IgG2a
- Reactivity:Bovine
- Western Blot:Yes
- Size:100 μg
- Gene ID:3383
- Antigen Synonyms:ICAM-1|major group rhinovirus receptor|human rhinovirus receptor|BB2|cell surface glycoprotein P3.58|intercellular adhesion molecule 1|P3.58
- Storage Buffer:Phosphate buffered saline pH 7.4 containing 15mM sodium azide
- Storage Temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Tissue / cell preparation (Human). (Burkitt?s lymphoma cell line RAJI).
- Tested Applications:Fluorescence-Activated Cell Sorting
- Purification:>95% by SDS-PAGE
- Cat. No.:89361-182
- Supplier no.:GTX22213
Specifications
About this item
Human CD54, also known as intercellular adhesion molecule-1 (ICAM1), is a 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with CD50 (ICAM3) and with CD102 (ICAM2). ICAM1 (CD54) is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 (CD54) is detected in some carcinoma and melanoma cells. Soluble ICAM1 (CD54) is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
ELISA: Use at an assay dependant concentration. The antibody reacts with soluble ICAM-1 in human serum. FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions.FACS:2 ?g/ml.IHC-P:10 ?g/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: ICAM1
Clonality: Monoclonal
Clone: MEM-111
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Bovine