The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.

For ELISA: Use at a concentration of 0.5-1 ?g/ml. For FACS: Use 5?g (undiluted) to label 10^6 clells - Incubate for 30min at 4oC. For ICC: Use at a dilution of 1-5 ?g/ml. For IHC-Fr: Use at a dilution of 1-5 ?g/ml. For IP: Use at a dilution of 1-2 ?g/ml. For WB: Use at a dilution of 1-5 ?g/ml. Predicted molecular weight: 160 kDa. Include 2mM Ca+2 with the antibody and avoid EDTA. Product GTX16897 has been reported to bind best to the unreduced LDL-R. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Type: Primary
Antigen: LDLR
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Bovine