Specifications
- Antibody Type:Primary
- Antigen Name:Breast Cancer type 1 susceptibility protein
- Antigen Symbol:BRCA1
- Clonality:Monoclonal
- Clone:MS13
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG1
- Reactivity:Human
- Western Blot:Yes
- Size:50 μg
- Gene ID:672
- Antigen Synonyms:BROVCA1|BRCA1/BRCA2-containing complex|subunit 1|PPP1R53|breast and ovarian cancer sususceptibility protein 1|RING finger protein 53|IRIS|protein phosphatase 1|breast cancer type 1 susceptibility protein|RNF53|PNCA4|regulatory subunit 53|breast and ovarian cancer susceptibility protein 1|PSCP|BRCAI|BRCC1
- Storage Buffer:0.05 M sodium phosphate buffer, 0.2% gelatin, with 0.1% sodium azide
- Molecular Weight:220 kDa
- Storage Temperature:Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
- Concentration:0.1 mg/ml
- Immunogen:Recombinant full length protein (Human).
- Tested Applications:ICC/IF, IHC-P, IP, WB
- Cat. No.:89351-244
- Supplier no.:GTX16781
Specifications
About this item
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase; phosphorylated BRCA1 resides in inner chromosomal structure, centrosome, cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein, participate in transcription coupled repair of oxidative DNA damage, X-chromosome inactivation, and can function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA1 protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.
For IF, IHC-P, and IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1.5 - 2.5 µg/ml. Predicted molecular weight: 207 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: BRCA1
Clonality: Monoclonal
Clone: MS13
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human