Gross cystic disease of the breast is benign premenopausal disorder in which cysts are a predominant pathological lesion. These cysts appear to be formed from excessive aporcine cystic secretions. This fluid is composed of several glycoproteins including a unique 15kD monomer protein, PIP. Cytosolic analysis of normal tissue specimens from all major organs has demonstrated PIP in aporcine epithelia, lacrimal, ceruminous and Molls glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands. PIP and prostate specific antigen are co-expressed in androgen receptor-positive breast tumors.

IHC-P: Use at a dilution of 1/15 - 1/30. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: PIP
Clonality: Monoclonal
Clone: 0.N.307
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human