Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.

IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 2 - 4 ?g/ml for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 minutesIP: Use 2?g/mg protein lysate. Use protein G. WB: Use at a concentration of 1 ?g/ml for 2 hours at room temperature. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: Stratifin
Clonality: Monoclonal
Clone: 1.N.6
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human