Mouse monoclonal [35509.11] to MCP2

ELISA: Use at a concentration of 0.5 - 1 µg/ml to detect <12.5ng/ml of recombinant human MCP2. Neut: Use at a concentration of 0.5 - 10 µg/ml. WB: Use at a concentration of 1 - 2 µg/ml to detect 50ng/lane of recombinant human MCP2 under reducing and non-reducing conditions. Predicted molecular weight: 9 kDa. Human MCP2 is tested for its ability to neutralize the chemotactic activity of rhMCP2 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP2 that is present at a concentration just high enough to elicit a maximum response. In this bioassay 0.4ug/ml rhMCP2 was mixed with various dilutions of the antibody for 30 minutes at 22¡C. After preincubation, 35ul of the antigen-antibody mixture were transferred to the lower compartment of a 96-well chemotaxis chamber. The chamber was reassembled, human monocytes were added to the top chamber and incubated at 37¡ for approximately 75 minutes in a humidified CO2 incubator. The chemotaxis filter was then fixed and stained. The optical density of the filter is proportional to the number of cells that migrated across the filter. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: MCP2
Clonality: Monoclonal
Clone: 35509.11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human