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- Size:10 µg
- Cat. No.:102136-986
- Supplier no.:NBA1-07085
The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.
- Buffer: 5 mM Tris pH 8.0
- Specificity: pCMV-SD OLLAS DYKDDDDK Vector for the expression of secretory protein with N-terminally FLAG(DYKDDDDK) and OLLAS dual tandem tagged.
- Tested applications: Western Blot
Application Notes: Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants.
Alternate Names for SD OLLAS/DYKDDDDK Vector:
DDDDK vector
DYKDDDDK epitope tag vector
DYKDDDDK vector
ECS tag vector
ECS vector
Enterokinase Cleavage Site epitope tag vector
Enterokinase Cleavage Site tag vector
FLAG vector (FLAG is a trademark of Sigma Aldrich)
OLLA-2 vector
xxxDDDDK epitope tag vector