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Specifications
- Size:10 µg
- Storage Temperature:Store at -20 °C. Avoid freeze-thaw cycles.
- Cat. No.:102136-982
- Supplier no.:NBA1-07083
Specifications
About this item
The pCMV-N-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with an N-terminal OLLAS tag. Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.
- Buffer: 5 mM Tris pH 8.0
- Specificity: pCMV-N OLLAS Vector for the intracellular expression of N-terminally OLLAS-tagged protein.
- Tested applications: Western Blot
Application Notes: Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown on the vector map above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants.