The Total RNA Mini and Maxi Tissue Kits are specially designed for purification of total RNA from a variety of animal tissues or cells
The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube. The method uses a detergent and chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed within 30 minutes for the Mini Kit, and 60 minutes for the Maxi Kit. The purified RNA is ready for RT-PCR, Northern blotting, primer extension, and cDNA library construction.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
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